Cloning Services

1. PCR Cloning from Plasmid Templates

    a) Design and synthesis of primers for amplification and sequence analysis

    b) Amplification of target sequence by PCR

    c) Subcloning into client-provided plasmid vector

    d) Preparation of Plasmid DNA

    e) Verification of DNA sequence of insert and junction regions

2. PCR Cloning from cDNA Templates

    a) Design and synthesis of primers for gene amplification and sequence analysis

    b) Amplification of target sequence from commercial source of cDNA by PCR

    c) Subcloning into client-provided plasmid vector

    d) Preparation of Plasmid DNA

    e) Verification of DNA sequence of insert and junction regions

3. PCR Mutagenesis

    a) Design and synthesis of primers for PCR mutagenesis and sequence analysis

    b) Amplification of fragment to introduce mutation by PCR

    c) Subcloning mutagenic fragment into original plasmid

    d) Preparation of Plasmid DNA

    e) Verification of DNA sequence of insert and junction regions

Deliverables

1. ~50 – 250 ug plasmid DNA

2. E. coli strain containing plasmid construct

3. Sequence chromatograms and data alignment

4. Construct map

5. Project report and data

Pricing

PCR Cloning from Plasmid Templates

Insert Size (No. Base Pairs)	Price per Construct (1 to 4)	Price per Construct (5 to 9)
Up to 2000	$1000	$850
2000 – 3000	$1100	$935
3000 – 4000	$1200	$1020
4000 – 5000	$1300	$1105
Over 5000	Inquire	Inquire

 PCR Mutagenesis

Insert Size (No. Base Pairs)	Price per Construct (1 to 4)	Price per Construct (5 to 9)
Up to 2000	$1200	$1020
2000 – 3000	$1300	$1105
3000 – 4000	$1400	$1190
4000 – 5000	$1500	$1275
Over 5000	Inquire	Inquire

 PCR Cloning from cDNA Templates

Insert Size (No. Base Pairs)	Price per Construct (1 to 4)	Price per Construct (5 to 9)
Up to  2000	$1500	$1275
2000 – 3000	$1600	$1360
3000 – 4000	$1700	$1445
4000 – 5000	$1800	$1530
Over 5000	Inquire	Inquire