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Bovine IFN alpha A Do-It-Yourself ELISA, ≤10 Plates

DIY0663B-003
$770.00
In Stock
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Bovine IFNα A ELISA Data

Bovine IFNα A Standard Curve

Bovine IFNα A ELISA Kit Components

Component Use Quantity Catalog #
Anti-Bovine IFNαA Polyclonal Antibody Capture Antibody 100 µg KP1840B-100
Biotinylated Anti-Bovine IFNαA Polyclonal Antibody Detection Antibody 50 µg KPB1841B-050
Bovine IFNαA Recombinant Protein Standard 5 µg RP0008B-005

 

Bovine IFNα A ELISA Specifications

The Bovine IFNαA Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine IFNαA ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately. 

For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.

IFNα Background

Type I interferons comprise a vast and growing group of IFN proteins. Homologous molecules to type I IFNs are found in many species, including all mammals, and some have been identified in birds, reptiles, amphibians and fish species. The mammalian types are designated IFN-α, IFN-β, IFN-κ, IFN-δ, IFN-ε, IFN-τ, IFN-ω, and IFN-ζ (also known as limitin). They are mainly involved in innate immune response against viral infection.

Catalog No.:
DIY0663B-003
Quantity:
1 Pack
Country of Origin:
USA
Applications:
Measurement of Bovine IFN alpha A in an ELISA.

32888966

A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus.

Sajiki Y, Konnai S, Okagawa T, Maekawa N, Nakamura H, Kato Y, Suzuki Y, Murata S, Ohashi K.

Dev Comp Immunol. 2020 Sep 2;114:103847. doi: 10.1016/j.dci.2020.103847.

Applications: Measurement of bovine TNF alpha, IFN alpha, and IFN gamma in culture supernatants by ELISA


32411730

Increased Susceptibility of Cattle to Intranasal RVFV Infection.

Kroeker AL, Smid V, Embury-Hyatt C, Collignon B, Pinette M, Babiuk S, Pickering B.

Front Vet Sci. 2020 Apr 29;7:137. doi: 10.3389/fvets.2020.00137. eCollection 2020.

Applications: Measurement of bovine IFN alpha and IFN beta in nasal and oral swabs by ELISA.

Abstract

Rift Valley Fever virus (RVFV) is a zoonotic mosquito-borne virus that belongs to the Phenuiviridae family. Infections in animal herds cause abortion storms, high mortality rates in neonates, and mild to severe symptoms. Infected animals can also transmit the virus to people, particularly people who live or work in close contact with livestock. There is currently an ongoing effort to produce safe and efficacious veterinary vaccines against RVFV in livestock to protect against both primary infection in animals and zoonotic infections in people. To test the efficacy of these vaccines it is essential to have a reliable challenge model in relevant target species, including ruminants. In this study we evaluated three routes of inoculation (intranasal, intradermal and a combination of routes) in Holstein cattle using an infectious dose of 107 pfu/ml and a virus strain from the 2006–2007 outbreak in Kenya and Sudan. Our results demonstrated that all routes of inoculation were effective at producing viremia in all animals; however, the intranasal route induced the highest levels and longest duration of viremia, the most noticeable clinical signs, and the most widespread infection of tissues. We therefore recommend using the intranasal inoculation for future vaccine and challenge studies.


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