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CartSwine IL-17A Do-It-Yourself ELISA, ≤10 Plates
Swine IL-17A ELISA Data
Swine IL-17A ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Swine IL-17A Polyclonal Antibody | Capture Antibody | 100 µg | KP0498S-100 |
| Biotinylated Anti-Swine IL-17A Polyclonal Antibody | Detection Antibody | 50 µg | KPB0499S-050 |
| Swine IL-17A Recombinant Protein | Standard | 5 µg | RP0128S-005 |
Swine IL-17A ELISA Specifications
The Swine IL-17A Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Swine IL-17A ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-17A Background
IL-17A is a member of the IL-17 family, which is comprised of 6 members [IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F]. IL-17 family members are involved in numerous immune regulatory functions, including inducing and mediating proinflammatory responses and allergic responses. IL-17 induces the production of many other cytokines (IL-6, G-CSF, GM-CSF, IL-1β, TGF-β, and TNF-α), chemokines, including IL-8 (CXCL8), GRO-α (CXCL1) and MCP-1 (CCL2) and prostaglandins from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes and macrophages).
Alternate Names - IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A
Deoxynivalenol as a new factor in the persistence of intestinal inflammatory diseases: an emerging hypothesis through possible modulation of Th17-mediated response.
Cano PM, Seeboth J, Meurens F, Cognie J, Abrami R, Oswald IP, Guzylack-Piriou L.
PLoS One. 2013;8(1):e53647. doi: 10.1371/journal.pone.0053647. Epub 2013 Jan 10.
Applications: Measurement of swine IL-17A in cell culture supernatants by ELISA
Abstract
BACKGROUND/AIMS:
Deoxynivalenol (DON) is a mycotoxin produced by Fusarium species which is commonly found in temperate regions worldwide as a natural contaminant of cereals. It is of great concern not only in terms of economic losses but also in terms of animal and public health. The digestive tract is the first and main target of this food contaminant and it represents a major site of immune tolerance. A finely tuned cross-talk between the innate and the adaptive immune systems ensures the homeostatic equilibrium between the mucosal immune system and commensal microorganisms. The aim of this study was to analyze the impact of DON on the intestinal immune response.
METHODOLOGY:
Non-transformed intestinal porcine epithelial cells IPEC-1 and porcine jejunal explants were used to investigate the effect of DON on the intestinal immune response and the modulation of naive T cells differentiation. Transcriptomic proteomic and flow cytometry analysis were performed.
RESULTS:
DON induced a pro-inflammatory response with a significant increase of expression of mRNA encoding for IL-8, IL-1α and IL-1β, TNF-α in all used models. Additionally, DON significantly induced the expression of genes involved in the differentiation of Th17 cells (STAT3, IL-17A, IL-6, IL-1β) at the expenses of the pathway of regulatory T cells (Treg) (FoxP3, RALDH1). DON also induced genes related to the pathogenic Th17 cells subset such as IL-23A, IL-22 and IL-21 and not genes related to the regulatory Th17 cells (rTh17) such as TGF-β and IL-10.
CONCLUSION:
DON triggered multiple immune modulatory effects which could be associated with an increased susceptibility to intestinal inflammatory diseases.
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