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Equine IFN beta 1 ELISA
- Catalog Number:
- DIY1005E
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Equus caballus (horse)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1002E-050 | |
| Standard | 5 µg | RP0935E-005 | |
| Capture Antibody | 100 µg | KP1001E-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1001E-100 | 1 Pack (<= 20 Plates) |
- The Equine IFN-β1 (Interferon Beta 1) ELISA is designed for the quantitative measurement of IFN-β1 in cell culture supernatants from horses and can also be used to measure IFN-β1 in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, cerebrospinal fluid, bronchoalveolar lavage fluid, and tissue lysates from organs such as lung, liver, spleen, kidney, and lymph nodes, supporting a broad range of immunology, antiviral, and translational research applications. IFN-β1 is a type I interferon primarily produced by fibroblasts, epithelial cells, and plasmacytoid dendritic cells, and it plays a central role in initiating antiviral responses, activating innate immunity, and regulating adaptive immune signaling. In the equine model, IFN-β1 is widely studied in the context of viral infections, vaccine responses, and inflammatory disorders, where its expression reflects host antiviral defense and immune activation. Measurement of IFN-β1 provides valuable insight into innate immune activation, disease progression, and the efficacy of immunomodulatory or antiviral therapies. Reported measurements of equine IFN-β1 have detected basal serum and plasma levels of approximately 20-250 pg/mL, with elevated levels-often 300-2,000 pg/mL-following viral infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from infected or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Equine Interferon-Beta (IFN-β) is a type I interferon produced primarily by virus-infected epithelial cells, fibroblasts, macrophages, and dendritic cells in horses (Equus caballus), where it serves as a key early mediator of innate antiviral immunity. Following recognition of viral nucleic acids by pattern recognition receptors such as RIG-I-like receptors and the cGAS-STING pathway, equine IFN-β is rapidly induced and signals through the type I interferon receptor complex (IFNAR1/IFNAR2), activating JAK/STAT signaling and promoting expression of interferon-stimulated genes (ISGs) that suppress viral replication, enhance antigen presentation, and stimulate natural killer (NK) cell and adaptive immune responses. IFN-β is critically involved in host defense against important equine viral pathogens including equine influenza virus, equine herpesvirus-1 and -4 (EHV-1/EHV-4), West Nile virus, and equine arteritis virus, and the timing and magnitude of IFN-β production can influence viral control, inflammation, and clinical outcome. Viral evasion or dysregulation of IFN-β signaling may contribute to persistent infection or immunopathology. In veterinary and translational research, characterization of equine IFN-β supports studies of respiratory and neurotropic viral pathogenesis, antiviral therapeutics, vaccine development, and comparative type I interferon biology in large-animal models relevant to both equine and human infectious disease research.
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