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Feline IL-8 ELISA
- Catalog Number:
- DIY1029F
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Felis catus (domestic cat)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | PBB0266S-050 | |
| Standard | 5 µg | RP0108F-005 | |
| Capture Antibody | 100 µg | PB0143S-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | PB0143S-100 | 1 Pack (<= 20 Plates) |
- The Feline IL-8 (Interleukin-8, also known as CXCL8) ELISA is designed for the quantitative measurement of IL-8 in cell culture supernatants from cats and can also be used to measure IL-8 in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, bronchoalveolar lavage fluid, synovial fluid, and tissue lysates from organs such as lung, spleen, liver, and lymph nodes, supporting a broad range of immunology, inflammation, and translational research applications. IL-8 is a chemokine primarily produced by macrophages, epithelial cells, and endothelial cells, and it plays a central role in recruiting neutrophils, promoting inflammatory responses, and coordinating innate immune defense. In the feline model, IL-8 is widely studied in the context of viral and bacterial infections, respiratory diseases, and inflammatory disorders, where its expression reflects immune activation and neutrophil recruitment. Measurement of IL-8 provides valuable insight into immune regulation, disease progression, and the efficacy of immunomodulatory interventions. Reported measurements of feline IL-8 have detected basal serum and plasma levels of approximately 15-200 pg/mL, with elevated levels-often 250-1,800 pg/mL-following infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from inflamed or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-8 in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Feline Interleukin-8 (IL-8), also known as CXCL8, is a potent pro-inflammatory chemokine that plays a central role in innate immune responses in cats (Felis catus) by promoting neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Produced by macrophages, epithelial cells, endothelial cells, and fibroblasts in response to microbial stimuli and inflammatory cytokines, feline IL-8 signals primarily through CXCR1 and CXCR2 receptors to activate intracellular pathways that drive leukocyte recruitment and amplification of acute inflammation. Elevated IL-8 expression is associated with numerous feline diseases, including feline infectious peritonitis (FIP), chronic gingivostomatitis, upper and lower respiratory infections, inflammatory bowel disease, and bacterial pneumonias, where excessive neutrophilic infiltration contributes to tissue pathology and disease severity. As a clinical biomarker, IL-8 supports assessment of inflammatory status and therapeutic response in veterinary medicine. Importantly, cats serve as valuable spontaneous animal models for human neutrophil-driven and chronic inflammatory diseases, and characterization of feline IL-8 enhances translational research in mucosal immunology, chronic oral inflammation, asthma-like airway disease, inflammatory bowel disease, and coronavirus-associated immunopathology. Because many feline conditions naturally mirror human inflammatory disorders, studying IL-8 signaling and regulation in cats provides insight into chemokine-mediated tissue damage, immune cell trafficking, and potential therapeutic strategies targeting CXCL8/CXCR pathways in both veterinary and human medicine.
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