Swine IFN beta ELISA

Catalog Number:: DIY1065S
Availability:: In stock
Application:: ELISA
100% Homology:: Phacochoerus africanus (Common warthog), Potamochoerus larvatus (bushpig), Potamochoerus porcus (red river hog), Sus scrofa (pig)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1064S-050
Standard	5 µg	RP0011S-005
Capture Antibody	100 µg	PB0124S-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	PB0124S-100	1 Pack (<= 20 Plates)

Product Description

The Swine IFN-β (Interferon Beta) ELISA is designed for the quantitative measurement of IFN-β in cell culture supernatants from pigs and can also be used to measure IFN-β in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, cerebrospinal fluid, bronchoalveolar lavage fluid, and tissue lysates from organs such as lung, liver, spleen, kidney, and lymph nodes, supporting a broad range of immunology, antiviral, and translational research applications. IFN-β is a type I interferon primarily produced by fibroblasts, epithelial cells, and plasmacytoid dendritic cells, and it plays a central role in initiating antiviral responses, activating innate immunity, and regulating adaptive immune signaling. In the swine model, IFN-β is widely studied in the context of viral infections, vaccine responses, and inflammatory disorders, where its expression reflects host antiviral defense and immune activation. Measurement of IFN-β provides valuable insight into innate immune activation, disease progression, and the efficacy of immunomodulatory or antiviral therapies. Reported measurements of swine IFN-β have detected basal serum and plasma levels of approximately 20-250 pg/mL, with elevated levels-often 300-2,000 pg/mL-following viral infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from infected or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IFN-β in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Background Info

Swine Interferon-Beta (IFN-β) is a type I interferon produced primarily by virus-infected epithelial cells, fibroblasts, macrophages, and dendritic cells in pigs (Sus scrofa domesticus), where it serves as a key early mediator of innate antiviral immunity. Following recognition of viral nucleic acids by pattern recognition receptors such as RIG-I, MDA5, and the cGAS-STING pathway, swine IFN-β is rapidly induced and signals through the type I interferon receptor complex (IFNAR1/IFNAR2), activating JAK/STAT signaling and driving expression of interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote activation of natural killer (NK) cells and adaptive immune responses. IFN-β plays a critical role in host defense against economically significant viral pathogens including porcine reproductive and respiratory syndrome virus (PRRSV), African swine fever virus (ASFV), swine influenza virus, classical swine fever virus (CSFV), and porcine epidemic diarrhea virus (PEDV). Many of these viruses possess mechanisms to suppress or delay IFN-β production, contributing to immune evasion, viral persistence, and disease severity. The timing and magnitude of IFN-β responses influence viral control, inflammation, and vaccine efficacy. In veterinary and translational research, characterization of swine IFN-β supports studies of antiviral immunity, immune evasion strategies, vaccine development, and comparative type I interferon biology in a large-animal model with physiological similarities to humans, enhancing relevance to both animal health and human infectious disease research.

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