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Bovine IL-2 ELISA
- Catalog Number:
- DIY1100B
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Bison bison bison (American buffalo), Bos gaurus (gaur), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle), Bubalus bubalis (water buffalo)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1099B-050 | |
| Standard | 5 µg | RP0026B-005 | |
| Capture Antibody | 100 µg | PB0121B-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | PB0121B-100 | 1 Pack (<= 20 Plates) |
- The Bovine IL-2 (Interleukin-2) ELISA is designed for the quantitative measurement of IL-2 in cell culture supernatants from cattle and can also be used to measure IL-2 in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, cerebrospinal fluid, and tissue lysates from organs such as lymph nodes, spleen, lung, liver, and intestine, supporting a broad range of immunology, inflammation, and translational research applications. IL-2 is a cytokine primarily produced by activated CD4⁺ T cells, and to a lesser extent by CD8⁺ T cells, and it plays a central role in stimulating T cell proliferation, supporting regulatory T cell function, and promoting immune system homeostasis. In the bovine model, IL-2 is especially important for evaluating cell-mediated immune responses to infection, vaccination, and autoimmune conditions, making it a critical biomarker for understanding cattle health, disease susceptibility, and immune competence. Measurement of IL-2 provides valuable insight into immune cell activation, the efficacy of vaccines or immunotherapies, and the progression of infectious or inflammatory diseases in cattle. Reported measurements of bovine IL-2 have detected basal serum and plasma levels of approximately 5-100 pg/mL, with elevated levels-often 150-1,200 pg/mL-following immune stimulation, infection, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-2 in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Bovine IL-2 (Interleukin-2) is a central T cell-derived cytokine that regulates lymphocyte proliferation, differentiation, and immune homeostasis in cattle (Bos taurus). IL-2 is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes following antigen recognition and costimulatory signaling, and it functions through the IL-2 receptor complex (IL-2Rα/CD25, IL-2Rβ, and the common γ chain) to promote clonal expansion of antigen-specific T cells, enhance cytotoxic T lymphocyte and natural killer (NK) cell activity, and support development and maintenance of regulatory T cells (Tregs). In healthy cattle, basal IL-2 expression is generally low but increases during infection, vaccination, and immune stimulation. Altered IL-2 responses have been associated with bovine viral diarrhea virus (BVDV), bovine leukemia virus (BLV), Mycobacterium bovis (bovine tuberculosis), mastitis, and bovine respiratory disease complex. Cattle serve as important natural models for human tuberculosis, retroviral infection, and mucosal immune responses, as well as for vaccine development and immunomodulatory therapies. In veterinary and translational research, bovine IL-2 is widely used as a biomarker of T cell activation and cell-mediated immunity and is studied in the context of host-pathogen interactions, vaccine efficacy, and immune regulation in livestock health.
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