Chicken IFN gamma Polyclonal Antibody

Anti-chicken IFN-γ polyclonal antibody (catalog PB0442C) is made in rabbits, affinity-purified by chicken IFN-γ affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant chicken IFN-γ (immunogen) and the calculated molecular weight of chicken IFN-γ is ~16.7 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 1-10 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0442C; standard protein RP0929C-005; detection antibody PBB0448C; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows no reactivity to bovine, canine, cynomolgus monkey, dolphin, equine, feline, human, mouse, ovine, rabbit, swine or zebrafish IFN-γ. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.

Chicken Interferon-Gamma (IFN-γ) is the sole type II interferon in chickens (Gallus gallus) and is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes as well as natural killer (NK)-like cells, where it plays a central role in cell-mediated (Th1-type) immunity. IFN-γ signals through the IFN-γ receptor complex (IFNGR1/IFNGR2), activating JAK/STAT1 pathways and inducing genes involved in macrophage activation, enhanced antigen presentation via upregulation of MHC class I and II molecules, and promotion of cytotoxic T-cell responses. In poultry health, IFN-γ is critical for defense against intracellular pathogens and economically significant diseases such as avian influenza virus, Newcastle disease virus, Marek's disease virus, Salmonella spp., Eimeria spp. (coccidiosis), and Mycobacterium avium, where robust Th1 responses contribute to pathogen control and vaccine-induced protection. Altered or insufficient IFN-γ responses can impact disease susceptibility, growth performance, and flock health. In research settings, chickens serve as important models for avian viral oncogenesis (Marek's disease), mucosal immunity, and vaccine development, and characterization of chicken IFN-γ supports studies of T-cell biology, host-pathogen interactions, immune correlates of protection, and comparative cell-mediated immunity relevant to both veterinary and broader immunological research.

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