Bovine CCL5 (RANTES) Recombinant Protein

Catalog Number:: RP0062B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos taurus (cattle), Bubalus bubalis (water buffalo), Bubalus kerabau (carabao)

Product Description

Bovine CCL5 (alias RANTES, Regulated on Activation, Normal T Cell Expressed and Secreted) (catalog RP0062B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.9 kDa, 68 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across cattle, water buffalo, and carabao. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration (particularly T cells, monocytes, eosinophils and NK cells); typical experimental uses include chemotaxis and cell-migration assays, receptor-binding and signaling studies, cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	SPYASDTTPC CFAYISRPLP RTHVQEYFYT SSKCSMAAVV FITRKNRQVC ANPEKKWVRE YINALELS (68)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	7.9 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine CCL5 (C-C motif chemokine ligand 5, also known as RANTES - Regulated upon Activation, Normal T cell Expressed and Secreted) is a proinflammatory chemokine that plays an important role in immune cell recruitment and inflammatory responses in cattle (Bos taurus). CCL5 belongs to the CC chemokine family, which includes related chemokines such as CCL3 (MIP-1α), CCL4 (MIP-1β), and CCL2 (MCP-1) that regulate leukocyte trafficking during immune activation. In cattle, CCL5 is produced primarily by activated T lymphocytes, macrophages, dendritic cells, platelets, and epithelial cells in response to infection, inflammatory stimuli, or tissue injury. CCL5 exerts its biological effects mainly through binding to the chemokine receptors CCR1, CCR3, and CCR5, triggering intracellular signaling pathways that promote chemotaxis and activation of T cells, monocytes, macrophages, eosinophils, and natural killer (NK) cells at sites of infection or inflammation. In bovine health, CCL5 contributes to immune responses against bacterial and viral pathogens, including those involved in mastitis, respiratory disease complexes, and viral infections such as bovine viral diarrhea virus (BVDV). By promoting recruitment and activation of immune cells, CCL5 helps coordinate local inflammatory responses and antiviral defense mechanisms. Because chemokine-mediated immune cell trafficking is essential for effective host defense, bovine CCL5 is studied in veterinary immunology, infectious disease research, and mastitis biology, contributing to improved understanding of immune regulation and disease resistance in cattle.

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