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Canine CCL5 (RANTES) Recombinant Protein
- Catalog Number:
- RP0454D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine CCL5 (RANTES) (catalog RP0454D) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.9 kDa, 68 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL5/RANTES signaling and immune-cell chemotaxis (including recruitment and activation of T cells, eosinophils, and monocytes); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SPYASDTTPC CFAYISGRLP FTHVQEYFYT SSKCSMPAVV FVTRKHRQVC ANPQKKWVRE YINSLEMS (68) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.9 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine CCL5 (C-C motif chemokine ligand 5, also known as RANTES - Regulated upon Activation, Normal T cell Expressed and Secreted) is a proinflammatory chemokine that regulates immune cell recruitment, inflammatory signaling, and antiviral responses in dogs (Canis lupus familiaris). CCL5 belongs to the CC chemokine family, which also includes CCL3 (MIP-1α), CCL4 (MIP-1β), and CCL2 (MCP-1), chemokines that control leukocyte trafficking during immune activation. In dogs, CCL5 is produced primarily by activated T lymphocytes, macrophages, dendritic cells, platelets, and epithelial cells in response to infection, inflammatory stimuli, or tissue injury, and exerts its biological effects mainly through binding to the chemokine receptors CCR1, CCR3, and CCR5, promoting chemotaxis and activation of T cells, monocytes/macrophages, eosinophils, and natural killer (NK) cells at sites of inflammation. CCL5 contributes to immune responses against viral and bacterial pathogens and is involved in chronic inflammatory and immune-mediated diseases such as canine atopic dermatitis and inflammatory bowel disease. Because dogs naturally develop several diseases that resemble human conditions, including allergic skin disease, inflammatory bowel disease, and certain cancers, canine CCL5 is also studied in comparative animal models of human inflammatory and immune-mediated disorders, helping to understand chemokine-driven leukocyte recruitment, tumor microenvironment signaling, and therapeutic targeting of the CCL5-CCR5 pathway.
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