Bovine TNF alpha ELISA

Catalog Number:: DIY0675B
Availability:: In stock
Application:: ELISA
100% Homology:: Bison bison bison (American buffalo), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle), Bubalus bubalis (water buffalo), Bubalus kerabau (carabao)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	PBB0278B-050
Standard	5 µg	RP0055B-005
Capture Antibody	100 µg	PB0275B-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	PB0275B-100	1 Pack (<= 20 Plates)

Product Description

The Bovine TNF-α (Tumor Necrosis Factor-alpha) ELISA is designed for the quantitative measurement of bovine TNF-α in cell culture supernatants. With minor modifications to the assay procedure, this ELISA may also be used to measure TNF-α in additional biological sample types reported in the literature, including serum, plasma, whole blood, peripheral blood mononuclear cell (PBMC) culture supernatants, tissue homogenates or lysates, milk, colostrum, bronchoalveolar lavage fluid (BALF), and synovial fluid, depending on the experimental model and disease context. This flexibility supports a wide range of immunology and translational research applications. Tumor Necrosis Factor-alpha (TNF-α) is a pro-inflammatory cytokine primarily produced by macrophages, monocytes, T cells, and natural killer (NK) cells. It plays a central role in immune regulation, inflammation, apoptosis, and host defense against infection. Measurement of TNF-α is widely used to assess inflammatory responses, immune activation, infection progression, and tissue injury in bovine and related species. TNF-α serves as an important biomarker of systemic inflammation, immune-mediated disease, and vaccine responses, supporting studies in animal health, comparative immunology, and translational research. TNF-α is a member of the TNF superfamily, which includes other related cytokines such as TNF-β (lymphotoxin-alpha), TRAIL, FasL, and CD40L. These cytokines share structural features and function in immune regulation, apoptosis, and inflammatory signaling, with TNF-α being a key mediator of acute and chronic inflammatory responses. The bovine TNF-α protein is 100% homologous to American Buffalo (Bubalus bubalis), Banteng (Bos javanicus), Carabao (Bubalus carabanensis), Water Buffalo (Bubalus bubalis), Yak (Bos grunniens), and Zebu (Bos indicus) TNF-α, making this ELISA well suited for studies involving these species and closely related animals where sequence identity supports cross-species detection. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Background Info

Bovine Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in cattle (Bos taurus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, molecules involved in regulating immune signaling, apoptosis, and inflammatory processes. In cattle, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In bovine health, TNF-α plays an important role in immune responses to bacterial infections such as mastitis caused by Escherichia coli or Staphylococcus aureus, as well as mycobacterial infections including bovine tuberculosis (Mycobacterium bovis), where it contributes to macrophage activation and granuloma formation. TNF-α is also involved in systemic inflammatory responses and metabolic changes during infection or stress. Because TNF-α is a key regulator of inflammation and host defense, bovine TNF-α is widely studied in veterinary immunology, infectious disease research, and mastitis biology, contributing to improved understanding of immune responses and inflammatory regulation in cattle.

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