Bovine VEGF-A ELISA

Catalog Number:: DIY0676B
Application:: ELISA
100% Homology:: Bison bison bison (American buffalo), Bos taurus (cattle), Bubalus bubalis (water buffalo), Bubalus kerabau (carabao)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	PBB0279B-050
Standard	5 µg	RP0072B-005
Capture Antibody	100 µg	PB0276B-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	PB0276B-100	1 Pack (<= 20 Plates)

Product Description

The Bovine VEGF-A (Vascular Endothelial Growth Factor A) ELISA is designed for the quantitative measurement of bovine VEGF-A in cell culture supernatants. With minor modifications to the assay procedure, this ELISA may also be used to measure VEGF-A in additional biological sample types reported in the literature, including serum, plasma, whole blood, tissue homogenates or lysates, milk, colostrum, bronchoalveolar lavage fluid (BALF), and synovial fluid, depending on the experimental model and physiological context. This flexibility supports a wide range of immunology, angiogenesis, and translational research applications. Reported measurements: VEGF-A has been detected in bovine serum (~0.2-1.5 ng/mL), plasma (~0.05-0.4 ng/mL), tissue homogenates or lysates (~0.5-3 ng/mL), milk (~0.1-0.3 ng/mL), colostrum (~0.2-0.6 ng/mL), and synovial fluid (~0.1-0.5 ng/mL), although concentrations may vary depending on health status, experimental conditions, and sample handling. Vascular Endothelial Growth Factor A (VEGF-A) is a key regulator of angiogenesis, vasculogenesis, and vascular permeability. It is primarily produced by endothelial cells, fibroblasts, macrophages, and other stromal cells in response to hypoxia or inflammatory stimuli. Measurement of VEGF-A is widely used to assess vascular growth, tissue remodeling, wound healing, tumor angiogenesis, and inflammatory responses in bovine and related species. Why measure VEGF-A: VEGF-A is a critical biomarker for vascular activation, tissue regeneration, and disease progression. Changes in VEGF-A levels can indicate angiogenic responses, inflammatory or hypoxic stress, and the effectiveness of therapeutic interventions. In livestock and comparative immunology research, VEGF-A measurement provides insights into tissue health, immune-mediated vascular changes, and pathological conditions such as mastitis, wound healing, or tumor growth. Accurate quantification of VEGF-A enables researchers to correlate vascular and immune responses with experimental or clinical outcomes, improving understanding of animal health and disease mechanisms. VEGF-A is a member of the VEGF family of growth factors, which includes VEGF-B, VEGF-C, VEGF-D, and placental growth factor (PlGF). These growth factors share structural features and function in angiogenesis, lymphangiogenesis, and endothelial cell signaling, with VEGF-A being a primary mediator of blood vessel formation and vascular remodeling. The bovine VEGF-A protein is 100% homologous to American Buffalo (Bubalus bubalis), Banteng (Bos javanicus), Carabao (Bubalus carabanensis), Water Buffalo (Bubalus bubalis), Yak (Bos grunniens), and Zebu (Bos indicus) VEGF-A, making this ELISA well suited for studies involving these species and closely related animals where sequence identity supports cross-species detection. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties/Specifications

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Bovine Vascular Endothelial Growth Factor A (VEGF-A) is a member of the vascular endothelial growth factor (VEGF) family, which includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, and placental growth factor (PlGF)—key regulators of angiogenesis and vascular development. In cattle (Bos taurus), VEGF-A is produced by multiple cell types including endothelial cells, macrophages, fibroblasts, epithelial cells, and trophoblast cells, particularly in response to hypoxia, inflammation, and tissue injury. VEGF-A exerts its biological effects mainly by binding to VEGF receptor-1 (VEGFR-1/Flt-1) and VEGF receptor-2 (VEGFR-2/KDR) on endothelial cells, activating intracellular signaling pathways such as MAPK/ERK, PI3K/AKT, and PLCγ, which promote endothelial cell proliferation, migration, vascular permeability, and formation of new blood vessels. In bovine physiology, VEGF-A plays critical roles in placental vascular development, reproductive function, mammary gland development, and tissue repair. It is particularly important during pregnancy and placentation, where VEGF-mediated angiogenesis supports maternal–fetal blood supply and nutrient exchange. VEGF-A is also involved in wound healing and inflammatory responses, contributing to vascular remodeling and tissue regeneration. Because angiogenesis is essential for reproductive efficiency, mammary gland function, and tissue repair in cattle, bovine VEGF-A is widely studied in reproductive biology, placental development, vascular biology, and livestock health research.