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Canine IL-2 ELISA
- Catalog Number:
- DIY0680D
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Nyctereutes procyonoides (raccoon dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)
Powered by Bioz Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | PBB0268D-050 | |
| Recombinant Protein Standard | 5 µg | RP0129D-005 | |
| Capture Antibody | 100 µg | PB0155D-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | PB0155D-100 | 1 Pack (<= 20 Plates) |
- The Canine IL-2 (Interleukin-2) ELISA is designed for the quantitative measurement of canine IL-2 in cell culture supernatants. With minor modifications to the assay procedure, this ELISA may also be used to measure IL-2 in additional biological sample types reported in the literature, including serum, plasma, whole blood, peripheral blood mononuclear cell (PBMC) culture supernatants, and tissue homogenates or lysates, depending on the experimental model and physiological context. This flexibility supports a wide range of immunology and translational research applications. Reported measurements: Canine IL-2 has been detected in serum (~10-150 pg/mL), plasma (~5-100 pg/mL), and PBMC culture supernatants (~50-1000 pg/mL under stimulation conditions), although levels vary depending on immune activation, infection, or experimental treatment. Interleukin-2 (IL-2) is a cytokine primarily produced by activated T lymphocytes. It plays a central role in T-cell proliferation, differentiation, survival, and the regulation of immune responses, including the expansion of regulatory T cells and NK cell activation. Measurement of IL-2 is widely used to assess adaptive immune activation, T-cell function, vaccine response, and immunotherapy efficacy in canine and related species. IL-2 serves as an important biomarker of cell-mediated immunity and immune system functionality, supporting studies in veterinary immunology, comparative immunology, and translational research. IL-2 is a member of the common gamma-chain (γc) cytokine family, which also includes IL-4, IL-7, IL-9, IL-15, and IL-21. These cytokines share structural and functional features, including roles in lymphocyte development, proliferation, and immune regulation, while IL-2 uniquely promotes T-cell clonal expansion and survival. The canine IL-2 protein is 100% homologous to Arctic Fox (Vulpes lagopus), Dingo (Canis dingo), Raccoon Dog (Nyctereutes procyonoides), and Red Fox (Vulpes vulpes) IL-2, making this ELISA well suited for studies involving these species and closely related animals where sequence identity supports cross-species detection. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Canine IL-2 (Interleukin-2) is a central T cell–derived cytokine that regulates lymphocyte proliferation, differentiation, and immune homeostasis in dogs (Canis lupus familiaris). IL-2 is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes following antigen recognition and costimulatory signaling, and it functions through the IL-2 receptor complex (IL-2Rα/CD25, IL-2Rβ, and the common γ chain) to promote clonal expansion of antigen-specific T cells, enhance cytotoxic T lymphocyte and natural killer (NK) cell activity, and support development and maintenance of regulatory T cells (Tregs). In healthy dogs, basal IL-2 expression is typically low but increases during infection, vaccination, and immune stimulation. Altered IL-2 responses have been associated with canine distemper virus, leishmaniasis, chronic inflammatory diseases, and immune-mediated disorders. Dogs serve as important translational animal models for human lymphoma and other cancers, atopic dermatitis, autoimmune disease, and certain neuroinflammatory and infectious diseases. In veterinary and comparative medicine research, canine IL-2 is widely used as a biomarker of T cell activation and cell-mediated immunity and has been investigated as an immunotherapeutic agent in oncology and chronic infectious disease settings.
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