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Guinea Pig TNF alpha ELISA
- Catalog Number:
- DIY0709GP
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Cavia porcellus (domestic guinea pig)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | PBB0452GP-050 | |
| Standard | 5 µg | RP0345GP-005 | |
| Capture Antibody | 100 µg | PB0446GP-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | PB0446GP-100 | 1 Pack (<= 20 Plates) |
- The Guinea Pig TNF-α (Tumor Necrosis Factor Alpha) ELISA is designed for the quantitative measurement of guinea pig TNF-α in cell culture supernatants. With minor modifications to the assay procedure, this ELISA can also be used to measure TNF-α in additional biological sample types reported in the literature, including serum, plasma, and tissue homogenates, providing flexibility for a broad range of immunology, inflammation, and translational research applications. TNF-α is a central pro-inflammatory cytokine produced primarily by activated macrophages, monocytes, and T cells, and it plays a critical role in innate and adaptive immune responses. In guinea pig models, TNF-α is a key mediator of granuloma formation, macrophage activation, and cytokine cascade amplification, making it particularly important in studies of infectious disease, vaccine response, pulmonary inflammation, and immunopathology. Measurement of TNF-α provides valuable insight into inflammatory signaling, disease severity, and therapeutic efficacy in guinea pig models that are frequently used when murine systems do not adequately recapitulate human immune responses. Reported measurements of guinea pig TNF-α have detected low to mid-picogram per milliliter concentrations in serum and plasma under basal conditions, with substantially elevated levels-often tens to hundreds of picograms per milliliter-observed following immune stimulation, infection, or inflammatory challenge, as well as measurable increases in tissue homogenates from affected organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing investigators to optimize assay sensitivity and dynamic range based on their specific sample type and experimental objectives. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Guinea Pig Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in guinea pigs (Cavia porcellus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, proteins that regulate immune signaling, apoptosis, and inflammatory pathways. In guinea pigs, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In guinea pigs, TNF-α is particularly important in immune responses to intracellular bacterial infections such as Mycobacterium tuberculosis, for which guinea pigs serve as a well-established animal model of human tuberculosis due to their similar disease pathology and granuloma formation. TNF-α contributes to macrophage activation, granuloma maintenance, and containment of mycobacterial infection, although excessive TNF signaling can also contribute to tissue inflammation and pathology. Because guinea pigs are widely used in infectious disease, immunology, and tuberculosis research, characterization of guinea pig TNF-α supports studies of cytokine regulation, host-pathogen interactions, and inflammatory responses relevant to both veterinary and human health.
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