Slide 1 of 1 
Rat APRIL ELISA
- Catalog Number:
- DIY0717R
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Rattus norvegicus (Norway rat)
Powered by Bioz Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | PBB0481R-050 | |
| Standard | 5 µg | RP0383R-005 | |
| Capture Antibody | 100 µg | PB0472R-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | PB0472R-100 | 1 Pack (<= 20 Plates) |
- The Rat APRIL (A Proliferation-Inducing Ligand, TNFSF13) ELISA is designed for the quantitative measurement of rat APRIL in cell culture supernatants and can also be used to measure APRIL in additional biological sample types reported in the literature, including serum, plasma, and tissue lysates, providing flexibility for a wide range of immunology, B cell biology, and translational research applications. APRIL is a member of the tumor necrosis factor (TNF) superfamily and plays a central role in B cell survival, proliferation, class-switch recombination, and plasma cell maintenance. Through interactions with the receptors TACI and BCMA, APRIL contributes to humoral immune responses, antibody production, and immune homeostasis. In rat models, APRIL is studied in the context of autoimmune disease, chronic inflammation, cancer, and vaccine responses, where dysregulated APRIL signaling is associated with aberrant B cell activation and pathogenic antibody production. Measurement of APRIL provides valuable insight into B cell-driven immune mechanisms, immune regulation, and therapeutic modulation of humoral immunity. Reported measurements of rat APRIL have detected low picogram per milliliter concentrations in serum and plasma under steady-state conditions, with elevated levels observed following immune activation, inflammatory challenge, or disease progression, as well as measurable expression in tissue lysates from lymphoid organs such as spleen and lymph nodes. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Rat APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, is a member of the tumor necrosis factor (TNF) superfamily that plays an important role in B cell survival, plasma cell maintenance, and regulation of humoral immunity in rats (Rattus norvegicus). APRIL is produced primarily by monocytes, macrophages, dendritic cells, and certain epithelial and stromal cells, and it signals through the receptors TACI (TNFRSF13B) and BCMA (TNFRSF17) to promote B cell maturation, immunoglobulin class switching, and long-lived plasma cell persistence. In rats, APRIL contributes to mucosal immunity, IgA production, and maintenance of antibody-secreting cells within lymphoid tissues and bone marrow. Dysregulated APRIL expression has been implicated in autoimmune and inflammatory disease models, including experimental arthritis, lupus-like disease, and transplant rejection studies. In biomedical research, rat APRIL is of particular interest in models of antibody-mediated autoimmunity, chronic inflammation, and B cell–related malignancies, where modulation of APRIL signaling is investigated as a strategy to regulate pathological B cell activation and antibody production.
Citations
Please choose a size to see price
RELATED MEDIA
