Slide 1 of 1 
Swine Erythropoietin (EPO) ELISA
- Catalog Number:
- DIY1006S
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Sus scrofa (pig)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1004S-050 | |
| Standard | 5 µg | RP0938S-005 | |
| Capture Antibody | 100 µg | KP1003S-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1003S-100 | 1 Pack (<= 20 Plates) |
- The Swine EPO (Erythropoietin) ELISA is designed for the quantitative measurement of EPO in cell culture supernatants from pigs and can also be used to measure EPO in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as kidney, liver, and spleen, supporting a broad range of hematology, physiology, and translational research applications. EPO is a glycoprotein hormone primarily produced by renal interstitial fibroblasts and, to a lesser extent, the liver, and it plays a central role in stimulating erythroid progenitor cell proliferation and differentiation, regulating red blood cell production, and maintaining oxygen homeostasis. In the swine model, EPO is widely studied in the context of anemia, hypoxia, blood production, and regenerative medicine, where its expression reflects erythropoietic activity and oxygen-sensing mechanisms. Measurement of EPO provides valuable insight into hematopoietic function, disease progression, and the efficacy of therapeutic interventions targeting anemia or hypoxia. Reported measurements of swine EPO have detected basal serum and plasma levels of approximately 5-45 mIU/mL, with elevated levels-often 50-400 mIU/mL-following hypoxic challenge, blood loss, or pharmacological stimulation, and measurable expression has also been observed in tissue lysates from kidney and liver under stress or regenerative conditions. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring EPO in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Swine Erythropoietin (EPO) is a glycoprotein hormone belonging to the type I cytokine family that regulates erythropoiesis, the production of red blood cells. In pigs (Sus scrofa), erythropoietin is produced primarily by peritubular interstitial cells in the kidney, with additional production occurring in the liver, in response to hypoxia or reduced oxygen-carrying capacity of the blood. Swine EPO binds to the erythropoietin receptor (EPOR) on erythroid progenitor cells in the bone marrow, activating intracellular signaling pathways such as JAK2-STAT5, PI3K-AKT, and MAPK, which promote the survival, proliferation, and differentiation of erythroid precursor cells into mature red blood cells. In swine physiology, erythropoietin is essential for maintaining normal hematocrit levels and adequate oxygen delivery to tissues, particularly during growth, physiological stress, or blood loss. Because pigs share significant anatomical, physiological, and hematological similarities with humans, they are widely used as translational animal models for studying anemia, kidney disease, and hypoxia-related disorders, and swine models have been used to investigate EPO regulation and erythropoiesis in conditions such as renal anemia and critical illness, providing insights relevant to human hematology and therapeutic EPO use.
Please choose a size to see price
RELATED MEDIA
