Bovine IL-1 beta ELISA

Catalog Number:: DIY1111B
Availability:: In stock
Application:: ELISA
100% Homology:: Bos indicus (zebu), Bos javanicus (banteng), Bos taurus (cattle)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1110B-050
Standard	5 µg	RP0106B-005
Capture Antibody	100 µg	KP1109B-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1109B-100	1 Pack (<= 20 Plates)

Product Description

The Bovine IL-1β (Interleukin-1 Beta) ELISA is designed for the quantitative measurement of IL-1β in cell culture supernatants from cattle and can also be used to measure IL-1β in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, cerebrospinal fluid, synovial fluid, and tissue lysates from organs such as lung, spleen, liver, kidney, and lymph nodes, supporting a broad range of immunology, inflammation, and translational research applications. IL-1β is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and dendritic cells, and it plays a central role in initiating inflammatory responses, activating immune cells, and mediating fever and tissue repair. In the bovine model, IL-1β is widely studied as a key biomarker for immune activation during bacterial and viral infections, mastitis, respiratory diseases, and inflammatory disorders, making it critical for assessing cattle health, disease susceptibility, and immune competence. Measurement of IL-1β provides valuable insight into immune system activation, disease progression, and the efficacy of vaccines or immunomodulatory interventions. Reported measurements of bovine IL-1β have detected basal serum and plasma levels of approximately 5-150 pg/mL, with elevated levels-often 200-2,000 pg/mL-following infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from inflamed or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-1β in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Background Info

Bovine Interleukin-1 Beta (IL-1β) is a potent pro-inflammatory cytokine produced primarily by activated macrophages, monocytes, and dendritic cells in cattle (Bos taurus) in response to microbial components or tissue injury. Synthesized as an inactive precursor (pro-IL-1β), it requires cleavage by caspase-1 within the inflammasome complex to become biologically active, after which it binds to the IL-1 receptor (IL-1R1) and activates NF-κB and MAPK signaling pathways to promote fever, leukocyte recruitment, endothelial activation, and acute-phase protein production. In bovine health, IL-1β plays a central role in inflammatory responses associated with economically significant diseases such as mastitis, bovine respiratory disease (BRD), metritis, lameness, and enteric infections, where it contributes to both pathogen clearance and inflammatory tissue damage. Elevated IL-1β expression in the mammary gland is closely linked to neutrophil recruitment and severity of intramammary infection. As a biomarker of innate immune activation, bovine IL-1β is widely used in studies of vaccine responses, immune modulation, and disease pathogenesis. In veterinary and translational research, characterization of bovine IL-1β supports investigations into inflammasome biology, host-pathogen interactions, inflammatory regulation in large-animal systems, and development of strategies to mitigate excessive inflammation while preserving protective immunity.

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