Chicken IL-10 ELISA

Catalog Number:: DIY1118C
Availability:: In stock
Application:: ELISA
100% Homology:: Gallus gallus (chicken)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1117C-050
Standard	5 µg	RP0018C-005
Capture Antibody	100 µg	KP1116C-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1116C-100	1 Pack (<= 20 Plates)

Product Description

The Chicken IL-10 (Interleukin-10) ELISA is designed for the quantitative measurement of IL-10 in cell culture supernatants from chickens and can also be used to measure IL-10 in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, bronchoalveolar lavage fluid, and tissue lysates from organs such as spleen, lung, liver, and intestine, supporting a broad range of immunology, inflammation, and avian translational research applications. IL-10 is an anti-inflammatory cytokine primarily produced by T regulatory cells, macrophages, and dendritic cells, and it plays a central role in suppressing excessive immune responses, regulating inflammation, and maintaining immune homeostasis. In the chicken model, IL-10 is widely studied in the context of infectious diseases, vaccination responses, and inflammatory disorders, where its expression reflects the modulation of immune responses and the balance between pro- and anti-inflammatory signaling. Measurement of IL-10 provides valuable insight into immune regulation, disease progression, and the efficacy of immunomodulatory or vaccine interventions. Reported measurements of chicken IL-10 have detected basal serum and plasma levels of approximately 5-150 pg/mL, with elevated levels-often 200-1,200 pg/mL-following infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from inflamed or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-10 in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Background Info

Chicken Interleukin-10 (IL-10) is a key anti-inflammatory cytokine produced primarily by regulatory T cells, Th2-like CD4⁺ T cells, macrophages, dendritic cells, and certain B-cell populations in chickens (Gallus gallus), where it plays a central role in limiting excessive immune activation and maintaining immune homeostasis. IL-10 signals through the IL-10 receptor complex (IL-10R1/IL-10R2), activating JAK/STAT3 pathways that suppress pro-inflammatory cytokine production (including IL-1β, IL-6, and IFN-γ), reduce antigen presentation, and downregulate Th1-type cell-mediated responses. In poultry health, IL-10 is critically involved in modulating immune responses during infections such as avian influenza virus, Newcastle disease virus, infectious bursal disease virus, Marek's disease virus, Salmonella spp., and Eimeria (coccidiosis), where elevated IL-10 can help limit tissue damage but may also contribute to immune suppression and pathogen persistence. The balance between IL-10 and pro-inflammatory cytokines influences vaccine efficacy, disease resistance, and growth performance in commercial flocks. As a biomarker of immune regulation, chicken IL-10 is widely studied in vaccine development, genetic selection for disease resilience, and investigations of mucosal immunity. Characterization of chicken IL-10 supports research into cytokine network regulation, host-pathogen interactions, and comparative immune tolerance mechanisms relevant to both poultry production and broader vertebrate immunology.

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