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Bovine CCL3 (MIP-1 alpha) ELISA
- Catalog Number:
- DIY1143B
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Bison bison bison (American buffalo), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1142B-050 | |
| Standard | 5 µg | RP0974B-005 | |
| Capture Antibody | 100 µg | KP1141B-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1141B-100 | 1 Pack (<= 20 Plates) |
- The Bovine CCL3 (Chemokine (C-C motif) Ligand 3, also known as MIP-1α) ELISA is designed for the quantitative measurement of CCL3 in cell culture supernatants from cattle and can also be used to measure CCL3 in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, synovial fluid, bronchoalveolar lavage fluid, and tissue lysates from organs such as lung, spleen, liver, and lymph nodes, supporting a broad range of immunology, inflammation, and translational research applications. CCL3 is a chemokine primarily produced by macrophages, monocytes, and activated lymphocytes, and it plays a central role in recruiting neutrophils, monocytes, and T cells to sites of inflammation, coordinating innate and adaptive immune responses, and mediating inflammatory signaling. In the bovine model, CCL3 is widely studied in the context of bacterial and viral infections, mastitis, respiratory diseases, and inflammatory disorders, where its expression reflects immune activation and the recruitment of leukocytes to affected tissues. Measurement of CCL3 provides valuable insight into immune system activation, disease progression, and the efficacy of vaccines or immunomodulatory interventions. Reported measurements of bovine CCL3 have detected basal serum and plasma levels of approximately 10-300 pg/mL, with elevated levels-often 400-2,500 pg/mL-following infection, immune stimulation, or inflammatory challenge, and measurable expression has also been observed in tissue lysates from inflamed or immune-activated organs. The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring CCL3 in other sample types to ensure optimal sensitivity and accuracy. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to further adjust assay parameters based on their specific sample type and experimental requirements. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Bovine CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in cattle (Bos taurus). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, natural killer (NK) cells, dendritic cells, and activated T lymphocytes to sites of infection or tissue injury. In cattle, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL3-mediated immune cell recruitment plays an important role in host defense against pathogens involved in diseases such as bovine respiratory disease complex (BRDC), mastitis, and mycobacterial infections including Mycobacterium bovis. While CCL3-driven leukocyte trafficking supports pathogen clearance and initiation of immune responses, excessive or prolonged expression may contribute to inflammatory tissue damage and chronic inflammation. As a regulator of immune cell migration and inflammatory signaling, bovine CCL3 is widely studied in investigations of host-pathogen interactions, inflammatory disease mechanisms, and vaccine responses aimed at improving disease resistance and health in cattle.
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