Cynomolgus Monkey IL-17A ELISA

Catalog Number:: DIY1249Y
Application:: ELISA
100% Homology:: Macaca fascicularis (crab-eating macaque), Macaca nemestrina (pig-tailed macaque)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1248Y-050
Standard	5 µg	RP1031Y-005
Capture Antibody	100 µg	KP1247Y-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1247Y-100	1 Pack (<= 20 Plates)

Product Description

The Cynomolgus Monkey IL-17A (Interleukin-17A) ELISA is designed for the quantitative measurement of IL-17A in cell culture supernatants from cynomolgus monkeys (Macaca fascicularis) and can also be used to measure IL-17A in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as spleen, lymph nodes, lung, and colon. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-17A in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-17A is a pro-inflammatory cytokine produced primarily by activated Th17 cells, γδ T cells, and innate lymphoid cells and plays a central role in host defense against bacterial and fungal infections, mucosal immunity, and inflammatory processes. In the cynomolgus monkey animal model, IL-17A is widely studied in the context of infectious disease, vaccine development, inflammatory disorders, and immunomodulatory research, where its expression reflects Th17-mediated immune responses. Measurement of IL-17A provides valuable insight into immune activation, cytokine signaling, disease progression, and the efficacy of therapeutic or vaccine interventions, with reported levels in cynomolgus monkeys typically detected at low picogram-per-milliliter concentrations under basal conditions (~5-50 pg/mL in serum or plasma) and increasing to hundreds of picograms per milliliter during immune stimulation or inflammatory challenge, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant cynomolgus monkey IL-17A protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties/Specifications

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Cynomolgus monkey IL-17A (Interleukin-17A) is a pro-inflammatory cytokine belonging to the IL-17 family that plays a central role in host defense and immune-mediated inflammation in Cynomolgus monkeys (Macaca fascicularis). The IL-17A protein is highly conserved among non-human primates and is functionally comparable to that of Rhesus monkey (Macaca mulatta) and humans, supporting its relevance in translational research. IL-17A is produced primarily by Th17 CD4⁺ T cells, as well as by γδ T cells and innate lymphoid cells, in response to microbial infection and inflammatory stimulation. It acts on epithelial, endothelial, and stromal cells to induce expression of pro-inflammatory cytokines (e.g., IL-6, TNF-α), chemokines (such as CXCL8), and antimicrobial peptides, thereby promoting neutrophil recruitment and enhancing mucosal immunity. In healthy Cynomolgus monkeys, basal IL-17A levels are typically low, but concentrations increase during bacterial or fungal infection, inflammatory challenge, or immune activation. In preclinical and translational research, Cynomolgus monkey IL-17A is particularly relevant in models of autoimmune disease, inflammatory disorders, vaccine immunogenicity, and evaluation of biologics targeting the IL-17 pathway, providing insight into Th17-mediated immune responses and neutrophilic inflammation in non-human primate systems.