Cynomolgus Monkey CCL11 (Eotaxin-1) ELISA

Catalog Number:
DIY1263Y
Availability:
In stock
Application:
ELISA
100% Homology:
Macaca fascicularis (crab-eating macaque), Macaca nemestrina (pig-tailed macaque), Mandrillus leucophaeus (drill)

Components

UsageQuantityComponentSize
Detection Antibody50 µgKPB1262Y-050
Standard5 µgRP1081Y-005
Capture Antibody100 µgKP1261Y-1001 Pack (<= 10 Plates)
Capture Antibody100 µg X 2KP1261Y-1001 Pack (<= 20 Plates)
  • The Cynomolgus Monkey CCL11 (C-C motif chemokine ligand 11, also known as Eotaxin-1) ELISA is designed for the quantitative measurement of CCL11 in cell culture supernatants from cynomolgus monkeys (Macaca fascicularis) and can also be used to measure CCL11 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as lung, spleen, and lymph nodes. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring CCL11 in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. CCL11 is a chemokine primarily produced by activated epithelial cells, fibroblasts, monocytes, and endothelial cells, and it plays a central role in recruiting eosinophils and other immune cells to sites of allergic inflammation or infection. In the cynomolgus monkey animal model, CCL11 is widely studied in the context of allergic disease, asthma, parasitic infections, and immunomodulatory research, where its expression reflects type 2 (Th2) immune responses. Measurement of CCL11 provides valuable insight into immune activation, chemokine signaling, disease progression, and the efficacy of therapeutic or vaccine interventions, with reported levels in cynomolgus monkeys typically detected at low picogram-per-milliliter concentrations under basal conditions (~10-100 pg/mL in serum or plasma) and increasing to hundreds of picograms per milliliter following immune stimulation, allergic challenge, or infection, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant cynomolgus monkey CCL11 protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
Storage Conditions2°C to 8°C or -20°C
Country Of OriginUSA
Cynomolgus monkey CCL11 (C-C motif chemokine ligand 11), also known as eotaxin-1, is a member of the CC chemokine family that regulates eosinophil recruitment and type 2–associated immune responses in Cynomolgus monkeys (Macaca fascicularis). The CCL11 protein is highly conserved among non-human primates and is functionally comparable to that of Rhesus monkey (Macaca mulatta) and humans. CCL11 is produced primarily by epithelial cells, fibroblasts, endothelial cells, and activated immune cells in response to Th2-associated cytokines such as IL-4 and IL-13, and it signals predominantly through the CCR3 receptor to promote chemotaxis and activation of eosinophils, as well as certain Th2 lymphocytes and basophils. In healthy Cynomolgus monkeys, circulating CCL11 levels are typically low, but concentrations may increase during allergic inflammation, parasitic infection, airway hypersensitivity, or experimental immune challenge. In preclinical and translational research, Cynomolgus monkey CCL11 is particularly relevant in models of asthma, allergic disease, parasitology, and evaluation of immunomodulatory biologics targeting Th2 or eosinophil-associated pathways. Characterization of CCL11 in Cynomolgus monkeys provides valuable insight into eosinophilic inflammation, chemokine-driven leukocyte trafficking, and translational mechanisms underlying allergic and type 2–mediated immune responses.

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