Swine IFN lambda 3 (IL-28B) ELISA

Catalog Number:: DIY1297S
Application:: ELISA
100% Homology:: Phacochoerus africanus (Common warthog), Sus scrofa (pig)

Components

Usage	Quantity	Component	Size
Detection Antibody	50 µg	KPB1296S-050
Standard	5 µg	RP1202S-005
Capture Antibody	100 µg	KP1295S-100	1 Pack (<= 10 Plates)
Capture Antibody	100 µg X 2	KP1295S-100	1 Pack (<= 20 Plates)

Product Description

The Swine IL-28B (Interleukin-28B, also known as IFN-λ3) ELISA is designed for the quantitative measurement of IL-28B in cell culture supernatants from pigs (Sus scrofa) and can also be used to measure IL-28B in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as lung, spleen, liver, and lymph nodes. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-28B in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-28B is a type III interferon produced primarily by plasmacytoid dendritic cells, epithelial cells, and macrophages and plays a central role in antiviral defense, immune modulation, and regulation of mucosal immunity. In the swine animal model, IL-28B is widely studied in the context of viral infections, vaccine development, innate immune responses, and immunomodulatory research, where its expression reflects antiviral signaling and host defense mechanisms. Measurement of IL-28B provides valuable insight into immune activation, cytokine signaling, disease progression, and the efficacy of therapeutic or vaccine interventions, with reported levels in pigs typically detected at low picogram-per-milliliter concentrations under basal conditions (~15-120 pg/mL in serum or plasma) and increasing to 150-800 pg/mL during viral challenge, infection, or immune stimulation, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant swine IL-28B protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.

Properties/Specifications

Storage Conditions	2°C to 8°C or -20°C
Country Of Origin	USA

Swine interferon lambda 3 (IFN-λ3), also known as interleukin-28B (IL-28B), is a member of the type III interferon family and functions as a key antiviral cytokine that signals through the IFNLR1 (IL-28Rα)/IL-10R2 receptor complex, activating JAK/STAT pathways and inducing interferon-stimulated genes (ISGs) that restrict viral replication primarily at epithelial surfaces. In swine, IFN-λ3 plays a critical role in mucosal immunity within the respiratory and gastrointestinal tracts, major entry points for economically significant pathogens such as porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine deltacoronavirus (PDCoV). Because type III interferons generate localized antiviral protection with less systemic inflammation than type I interferons, swine IFN-λ3 is likely essential for controlling viral spread while preserving tissue integrity and growth performance. As respiratory and enteric viral diseases substantially impact global pork production, swine IFN-λ3 (IL-28B) is highly relevant for understanding antiviral defense mechanisms, improving vaccine strategies, and developing immunomodulatory approaches to enhance herd health and biosecurity.