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Rabbit IL-2 ELISA
- Catalog Number:
- DIY1345U
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Oryctolagus cuniculus (rabbit)
Powered by Bioz Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1344U-050 | |
| Standard | 5 µg | RP0140U-005 | |
| Capture Antibody | 100 µg | KP1343U-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1343U-100 | 1 Pack (<= 20 Plates) |
- The Rabbit IL-2 (Interleukin-2) ELISA is designed for the quantitative measurement of IL-2 in cell culture supernatants from rabbits (Oryctolagus cuniculus) and can also be used to measure IL-2 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as spleen, lymph nodes, and thymus. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-2 in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-2 is a key cytokine produced primarily by activated T cells and plays a central role in T cell proliferation, immune regulation, and activation of natural killer (NK) cells. In the rabbit animal model, IL-2 is widely studied in the context of vaccine development, immunomodulatory research, infection, and adaptive immune responses, where its expression reflects T cell-mediated immune activation. Measurement of IL-2 provides valuable insight into immune activation, cytokine signaling, disease progression, and the efficacy of therapeutic or vaccine interventions, with reported levels in rabbits typically detected at low picogram-per-milliliter concentrations under basal conditions (~10-120 pg/mL in serum or plasma) and increasing during immune challenge, infection, or immunostimulatory treatment, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant rabbit IL-2 protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Rabbit IL-2 (Interleukin-2) is a central T cell–derived cytokine that regulates lymphocyte proliferation, differentiation, and immune homeostasis in rabbits (Oryctolagus cuniculus). IL-2 is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes following antigen recognition and costimulatory signaling, and it functions through the IL-2 receptor complex (IL-2Rα/CD25, IL-2Rβ/CD122, and the common γ chain/CD132) to promote clonal expansion of antigen-specific T cells, enhance cytotoxic T lymphocyte and natural killer (NK) cell activity, and support development and maintenance of regulatory T cells (Tregs). In healthy rabbits, basal IL-2 expression is generally low but increases during infection, vaccination, and immune stimulation. Altered IL-2 responses have been observed in models of tuberculosis, atherosclerosis, ocular inflammation, autoimmune-like disease, and tumor immunology. Rabbits serve as valuable translational models for cardiovascular disease, infectious disease, and immunogenicity studies. In biomedical research, rabbit IL-2 is widely used as a biomarker of T cell activation and cell-mediated immunity and supports investigation of vaccine efficacy, immune regulation, and immunotherapeutic strategies.
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