Slide 1 of 1 
Rabbit IL-6 ELISA
- Catalog Number:
- DIY1392U
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Oryctolagus cuniculus (rabbit)
Powered by Bioz Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1391U-050 | |
| Standard | 5 µg | RP1281U-005 | |
| Capture Antibody | 100 µg | KP1390U-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1390U-100 | 1 Pack (<= 20 Plates) |
- The Rabbit IL-6 (Interleukin-6) ELISA is designed for the quantitative measurement of IL-6 in cell culture supernatants from rabbits (Oryctolagus cuniculus) and can also be used to measure IL-6 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as spleen, liver, lung, and lymph nodes. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-6 in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-6 is a pleiotropic cytokine produced primarily by activated macrophages, T cells, and endothelial cells and plays a central role in inflammation, immune regulation, hematopoiesis, and acute phase responses. In the rabbit animal model, IL-6 is widely studied in the context of infectious disease, inflammatory responses, vaccine development, and immunomodulatory research, where its expression reflects immune activation and inflammatory signaling. Measurement of IL-6 provides valuable insight into immune responses, cytokine signaling, disease progression, and the efficacy of therapeutic or vaccine interventions, with reported levels in rabbits typically detected at low nanogram-per-milliliter concentrations under basal conditions (~0.2-2 ng/mL in serum or plasma) and increasing during immune challenge, infection, or inflammatory stimulation, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant rabbit IL-6 protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Rabbit Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine and a central member of the IL-6 cytokine family—including IL-6, IL-11, IL-27, IL-31, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and cardiotrophin-like cytokine factor 1 (CLCF1)—which signal through receptor complexes containing the shared gp130 subunit to activate JAK/STAT and MAPK pathways. In rabbits (Oryctolagus cuniculus), IL-6 is produced by macrophages, monocytes, endothelial cells, and fibroblasts in response to infection, tissue injury, and immune stimulation, where it regulates acute-phase protein production, fever, leukocyte recruitment, and B- and T-cell differentiation. Elevated IL-6 expression is observed in response to bacterial infections (such as Pasteurella multocida), viral diseases including rabbit hemorrhagic disease virus (RHDV), parasitic infections, and inflammatory conditions, contributing to systemic inflammation and disease severity. Rabbits are also widely used as translational models for atherosclerosis, ophthalmic disease, osteoarthritis, and vaccine development, where IL-6 plays a role in inflammatory signaling and tissue pathology. As both a biomarker and a mediator of immune activation, rabbit IL-6 is important in veterinary health monitoring and in biomedical research focused on host-pathogen interactions, chronic inflammation, cardiovascular disease, and evaluation of immunomodulatory therapies.
Citations
Please choose a size to see price
RELATED MEDIA
