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Dolphin IL-6 ELISA
- Catalog Number:
- DIY1417P
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Globicephala melas (long-finned pilot whale), Lagenorhynchus obliquidens (Pacific white-sided dolphin), Tursiops truncatus (common bottlenose dolphin)
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| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1417P-050 | |
| Standard | 5 µg | RP0993P-005 | |
| Capture Antibody | 100 µg | KP1416P-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1416P-100 | 1 Pack (<= 20 Plates) |
- The Dolphin IL-6 (Interleukin-6) ELISA is designed for the quantitative measurement of IL-6 in cell culture supernatants from dolphins (e.g., Tursiops truncatus) and can also be used to measure IL-6 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as spleen, liver, lung, and lymph nodes. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-6 in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-6 is a pleiotropic cytokine produced primarily by activated macrophages, T cells, and endothelial cells and plays a central role in inflammation, immune regulation, hematopoiesis, and acute phase responses. In the dolphin animal model, IL-6 is widely studied in the context of infectious disease, immune response, inflammation, and environmental stress studies, where its expression reflects immune activation and inflammatory signaling. Measurement of IL-6 provides valuable insight into immune responses, cytokine signaling, disease progression, and the efficacy of therapeutic or environmental interventions, with reported levels in dolphins typically detected at low nanogram-per-milliliter concentrations under basal conditions (~0.2-2 ng/mL in serum or plasma) and increasing during immune challenge, infection, or inflammatory stimulation, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant dolphin IL-6 protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Dolphin Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine and a key member of the IL-6 cytokine family—including IL-6, IL-11, IL-27, IL-31, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and cardiotrophin-like cytokine factor 1 (CLCF1)—which signal through receptor complexes utilizing the shared gp130 subunit to activate JAK/STAT and MAPK pathways. In dolphins, particularly the bottlenose dolphin (Tursiops truncatus), IL-6 plays an important role in regulating acute-phase responses, leukocyte activation, B- and T-cell differentiation, and systemic inflammation in response to infectious, inflammatory, and environmental challenges. Elevated IL-6 expression has been associated with bacterial and viral infections, fungal disease, wound healing, and exposure to environmental stressors or pollutants, making it a valuable biomarker for immune activation and overall health assessment in both free-ranging and managed populations. In marine mammal health and comparative immunology research, characterization of dolphin IL-6 supports studies of host-pathogen interactions, stress physiology, and immune adaptation to marine environments, while also contributing to translational insights, as dolphins share similarities with humans in longevity, chronic disease susceptibility, and complex immune regulation.
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