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Swine IL-3 ELISA
- Catalog Number:
- DIY1423S
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Sus scrofa (pig)
Powered by Bioz Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1422S-050 | |
| Standard | 5 µg | RP1298S-005 | |
| Capture Antibody | 100 µg | KP1421S-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1421S-100 | 1 Pack (<= 20 Plates) |
- The Swine IL-3 (Interleukin-3) ELISA is designed for the quantitative measurement of IL-3 in cell culture supernatants from pigs (Sus scrofa) and can also be used to measure IL-3 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as bone marrow, spleen, lymph nodes, and peripheral blood leukocytes. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-3 in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-3 is a hematopoietic growth factor produced primarily by activated T cells, mast cells, and stromal cells and plays a key role in the proliferation, differentiation, and survival of myeloid progenitor cells, including basophils, eosinophils, monocytes, and dendritic cells. In the swine animal model, IL-3 is widely studied in the context of hematopoiesis, immune development, inflammatory responses, and translational immunology, where pigs serve as a valuable large-animal model due to their physiological and immunological similarities to humans. Measurement of IL-3 provides valuable insight into bone marrow activity, immune cell development, cytokine signaling, and the evaluation of immunomodulatory or hematopoietic therapies, with reported levels in pigs typically detected at low picogram-per-milliliter concentrations under basal conditions (~5-100 pg/mL in serum or plasma) and increasing during immune activation, inflammation, or experimental stimulation, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant swine IL-3 protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Swine interleukin-3 (IL-3) is a hematopoietic growth factor primarily produced by activated T cells and mast cells that supports the proliferation, differentiation, and survival of multipotent hematopoietic progenitors through signaling via the IL-3 receptor complex (IL-3Rα/CD123 and the common β chain), activating JAK/STAT, MAPK, and PI3K pathways. In swine health, IL-3 plays an important role in regulating myeloid cell development, including monocytes, macrophages, dendritic cells, basophils, and mast cells, thereby influencing innate immunity, inflammatory responses, and host defense against bacterial and viral infections relevant to production systems. Dysregulated IL-3 signaling may contribute to excessive inflammation, allergic-type responses, or altered immune cell dynamics during respiratory and enteric diseases that impact growth performance and herd productivity. Beyond its importance in livestock health, swine IL-3 is highly valuable in animal model research because pigs share close physiological, immunological, and hematopoietic similarities with humans, making them strong translational models for studying bone marrow biology, inflammatory disorders, sepsis, and immune cell development. As such, swine IL-3 supports both veterinary immunology applications and comparative biomedical research aimed at understanding hematopoiesis and immune regulation across species.
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