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Ovine IL-13 ELISA
- Catalog Number:
- DIY1453V
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Ovis aries (sheep)
Powered by Bioz Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1452V-050 | |
| Standard | 5 µg | RP1097V-005 | |
| Capture Antibody | 100 µg | KP1451V-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1451V-100 | 1 Pack (<= 20 Plates) |
- The Ovine IL-13 (Interleukin-13) ELISA is designed for the quantitative measurement of IL-13 in cell culture supernatants from sheep (Ovis aries) and can also be used to measure IL-13 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as lung, intestine, lymph nodes, spleen, and skin. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring IL-13 in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. IL-13 is a Th2-associated cytokine produced primarily by CD4⁺ T helper cells, innate lymphoid cells, mast cells, and eosinophils and plays a central role in type 2 immune responses, regulation of inflammation, mucus production, tissue remodeling, and fibrosis. In the ovine animal model, IL-13 is widely studied in the context of gastrointestinal nematode infection, allergic-type inflammation, respiratory disease, and mucosal immunity, where its expression reflects Th2-driven immune responses and host defense mechanisms. Measurement of IL-13 provides valuable insight into type 2 cytokine signaling, host-parasite interactions, disease progression, and the evaluation of vaccines or immunomodulatory interventions, with reported levels in sheep typically detected at low picogram-per-milliliter concentrations under basal conditions (~10-120 pg/mL in serum or plasma) and increasing during parasitic infection, allergen exposure, or inflammatory stimulation, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant ovine IL-13 protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
Ovine Interleukin-13 (IL-13) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells and other type 2 immune populations in sheep (Ovis aries), where it plays a central role in regulating mucosal immunity, alternative macrophage activation, and tissue remodeling. IL-13 signals through receptor complexes composed of IL-13Rα1 and IL-4Rα, activating JAK/STAT6 pathways that promote IgE-associated responses, mucus production, suppression of certain pro-inflammatory cytokines, and support of wound healing and fibrotic processes. In ovine health, IL-13 is particularly important in immune responses to gastrointestinal nematodes and other parasitic infections, where Th2 polarization facilitates parasite expulsion and intestinal repair. IL-13 may also influence immune regulation in respiratory disease, mastitis, and chronic viral infections such as ovine progressive pneumonia (maedi-visna) by shaping the Th1/Th2 balance. While protective in limiting excessive inflammation and supporting tissue repair, prolonged or dysregulated IL-13 expression can contribute to fibrosis and chronic inflammatory pathology. As a biomarker of type 2 immune activation, ovine IL-13 is valuable in studies of host–parasite interactions, vaccine development, and genetic selection for disease resilience. Characterization of ovine IL-13 supports veterinary research into cytokine network regulation, mucosal immunity, and strategies to improve flock health and productivity.
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