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Human GM-CSF ELISA
- Catalog Number:
- DIY1485H
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Homo sapiens (human)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1484H-050 | |
| Standard | 5 µg | RP0919H-005 | |
| Capture Antibody | 100 µg | KP1483H-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1483H-100 | 1 Pack (<= 20 Plates) |
- The Human GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) ELISA is designed for the quantitative measurement of GM-CSF in cell culture supernatants from humans (Homo sapiens) and can also be used to measure GM-CSF in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as lung, spleen, lymph nodes, and bone marrow. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when measuring GM-CSF in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. GM-CSF is a cytokine produced primarily by activated T cells, macrophages, endothelial cells, and fibroblasts and plays a central role in hematopoiesis, myeloid cell differentiation, immune activation, and inflammation. In human studies and translational research, GM-CSF is widely investigated in the context of infectious disease, autoimmune disorders, cancer immunotherapy, and vaccine response, where its expression reflects activation of myeloid and dendritic cell pathways. Measurement of GM-CSF provides valuable insight into immune signaling, inflammatory pathways, disease progression, and the evaluation of immunomodulatory or therapeutic interventions, with reported levels typically detected at low picogram-per-milliliter concentrations under basal conditions (~5-150 pg/mL in serum or plasma) and increasing during immune stimulation, infection, or inflammatory challenge, depending on sample type and experimental context. The kit contains a capture antibody, detection antibody, and recombinant human GM-CSF protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Human GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In humans (Homo sapiens), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells in response to infection, inflammatory cytokines, or immune stimulation. Human GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, and enhance their antigen-presenting and inflammatory functions. In human health, GM-CSF plays an important role in host defense against infections, regulation of inflammatory responses, and development of immune cells involved in adaptive immunity. Dysregulation of GM-CSF signaling has been associated with inflammatory and autoimmune diseases, including rheumatoid arthritis and pulmonary alveolar proteinosis. Because of its ability to stimulate immune cell development and activation, GM-CSF is widely studied and used in clinical immunotherapy and vaccine research, including GM-CSF-based cancer vaccines designed to enhance dendritic cell activation and anti-tumor immune responses.
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