Chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemotactic protein-1 (MCP-1), is a small cytokine belonging to the CC chemokine family. There are at least 27 distinct members of the C-C subgroup reported for mammals. They are characterized by two adjacent cysteines. CCL2 (MCP-1) recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. CCL2 (MCP-1) is implicated in pathogeneses of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis.
The Feline CCL2 (MCP-1) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Feline CCL2 (MCP-1) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Feline CCL2 (MCP-1) Do-It-Yourself ELISA can also be used to measure Cheetah and Puma CCL2.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Component | Usage | Quantity | Catalog # |
Anti-Human CCL2 (MCP-1) Polyclonal Antibody | Capture Antibody | 100 µg | KP1623H-100 |
Biotinylated Anti-Human CCL2 (MCP-1) Polyclonal Antibody | Detection Antibody | 50 µg | KPB1624H-050 |
Feline CCL2 (MCP-1) Recombinant Protein | Standard | 5 µg | RP1548F-005 |
Feline CCL2 ELISA Data
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