Slide 1 of 1 
Ovine IL-1 beta ELISA
- Catalog Number:
- DIY1755V
- Availability:
- In stock
- Application:
- ELISA
- 100% Homology:
- Ovis aries (sheep), Pseudois nayaur (bharal)
Components
| Usage | Quantity | Component | Size |
|---|---|---|---|
| Detection Antibody | 50 µg | KPB1754V-050 | |
| Standard | 5 µg | RP0656V-005 | |
| Capture Antibody | 100 µg | KP1753V-100 | 1 Pack (<= 10 Plates) |
| Capture Antibody | 100 µg X 2 | KP1753V-100 | 1 Pack (<= 20 Plates) |
- The Ovine IL-1 beta (IL-1β) ELISA is designed for the quantitative measurement of interleukin-1 beta (IL-1β) in ovine (sheep) cell culture supernatants and can also be used to measure IL-1β in additional biological sample types reported in the literature, including serum, plasma, bronchoalveolar lavage (BAL) fluid, and tissue lysates, with minor protocol modifications recommended to optimize performance across different matrices. IL-1β is a potent pro-inflammatory cytokine produced primarily by activated monocytes, macrophages, and other innate immune cells following inflammasome activation and caspase-1-mediated cleavage of pro-IL-1β, and it plays a central role in fever induction, leukocyte recruitment, endothelial activation, and amplification of inflammatory signaling pathways. In healthy sheep, circulating IL-1β levels are typically very low or undetectable (often <5 pg/mL in serum or plasma), but concentrations can increase to tens or hundreds of pg/mL in response to bacterial or viral infection, tissue injury, parturition-associated inflammation, respiratory disease, or experimental immune stimulation, depending on physiological and pathological conditions. In veterinary and translational research, ovine IL-1β serves as a key biomarker of innate immune activation, inflammasome activity, infectious disease progression, vaccine response, and inflammatory conditions relevant to both agricultural health and large-animal biomedical models. The ELISA kit contains a capture antibody, detection antibody, and recombinant ovine IL-1β protein standard validated for use together in an ELISA format; kit components are not supplied in matched quantities and may be purchased separately. This product is for research use only, not for use in diagnostic or therapeutic procedures, and is manufactured in the USA.
| Storage Conditions | 2°C to 8°C or -20°C |
| Country Of Origin | USA |
- Ovine Interleukin-1 Beta (IL-1β) is a potent pro-inflammatory cytokine produced primarily by activated macrophages, monocytes, and dendritic cells in sheep (Ovis aries) in response to infection, tissue injury, or inflammatory stimuli. Synthesized as an inactive precursor (pro-IL-1β), it is cleaved into its active form by caspase-1 within inflammasome complexes-most notably the NLRP3 inflammasome-and signals through the IL-1 receptor type 1 (IL-1R1), activating NF-κB and MAPK pathways that promote fever, leukocyte recruitment, endothelial activation, and acute-phase protein production. In ovine health, IL-1β plays a central role in inflammatory conditions associated with mastitis, pneumonia, foot rot, enteric infections, parasitic infestations, and viral diseases such as ovine progressive pneumonia (maedi-visna), where excessive IL-1β production can contribute to tissue damage and chronic inflammation. Elevated IL-1β expression is also observed in the mammary gland during intramammary infection and serves as a marker of disease severity. As a biomarker of innate immune activation, ovine IL-1β is important for evaluating vaccine responses and immune status in flock management. In veterinary and translational research, characterization of ovine IL-1β supports studies of inflammasome biology, host-pathogen interactions in small ruminants, inflammatory regulation, and development of targeted anti-inflammatory strategies relevant to both animal health and comparative immunology.
Please choose a size to see price
CITATIONS
Takahashi Y, Takahashi T, Usuda H, Carter S, Fee EL, Furfaro L, Chemtob S, Olson DM, Keelan JA, Kallapur S, Kemp MW. Pharmacological blockade of the interleukin-1 receptor suppressed Escherichia coli lipopolysaccharide-induced neuroinflammation in preterm fetal sheep. Am J Obstet Gynecol MFM. 2023 Nov;5(11):101124. doi: 10.1016/j.ajogmf.2023.101124. Epub 2023 Aug 18. PMID: 37597799.
Application: ELISA
Sample Type: Amniotic Fliuid, Fetal Lung Tissue