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Bovine CXCL11 (I-TAC) Do-It-Yourself ELISA, ≤10 Plates

DIY1906B-003
$700.00
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Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family. It is also commonly called Interferon-inducible T-cell alpha chemoattractant (I-TAC). There have been 17 different C-X-C chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the C-X-C motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive C-X-C chemokines specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. C-X-C chemokines that lack the ELR motif are chemoattractant for lymphocytes. CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC) are homologous, interferon-inducible members of the CXC chemokine family that lack the tripeptide structure/function motif Glu-Leu-Arg (ELR) important in neutrophil chemoattraction. As such, these interferon-inducible ELR- CXC chemokines signal through a common receptor, CXCR3, to facilitate selective recruitment of mononuclear leukocytes, natural killer cells, and plasmacytoid dendritic cells to sites of inflammation.

The Bovine CXCL11 (I-TAC) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CXCL11 (I-TAC) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately. 

The Bovine CXCL11 (I-TAC) Do-It-Yourself ELISA can also be used to measure Bison, Water Buffalo, Wild Yak, and Zebu CXCL11 (I-TAC).

For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.

Component Use Quantity Catalog #
Anti-Bovine CXCL11 (I-TAC) Polyclonal Antibody Capture Antibody 100 µg KP1904B-100
Biotinylated Anti-Bovine CXCL11 (I-TAC) Polyclonal Antibody Detection Antibody 50 µg KPB1905B-050
Bovine CXCL11 (I-TAC) Recombinant Protein Standard 5 µg RP0016B-005

 

Bovine CXCL11 ELISA Data

Bovine CXCL11 Standard Curve

 

 

Catalog No.:
DIY1906B-003
Quantity:
1 Pack
Alias:
I-TAC
Country of Origin:
USA
Applications:
Measurement of Bovine, Bison, Water Buffalo, Wild Yak, and Zebu CXCL11 in an ELISA.

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