Ferret CXCL6 ELISA

Catalog Number:
DIY1980FT
Availability:
In stock
Application:
ELISA
100% Homology:
Mustela lutreola (European mink), Mustela nigripes (black-footed ferret), Mustela putorius furo (domestic ferret)

Components

UsageQuantityComponentSize
Detection Antibody50 µgKPB1979FT-050
Standard5 µgRP1732FT-005
Capture Antibody100 µgKP1978FT-1001 Pack (<= 10 Plates)
Capture Antibody100 µg X 2KP1978FT-1001 Pack (<= 20 Plates)
  • The Ferret CXCL6 (C-X-C motif chemokine ligand 6, also known as GCP-2) ELISA is designed for the quantitative measurement of CXCL6 in ferret (Mustela putorius furo) cell culture supernatants and can also be used to measure CXCL6 in additional biological sample types reported in the literature, including serum, plasma, bronchoalveolar lavage (BAL) fluid, and tissue lysates, with minor protocol modifications recommended to optimize performance across different matrices. CXCL6 is a pro-inflammatory CXC chemokine produced by monocytes, macrophages, epithelial cells, endothelial cells, and fibroblasts in response to inflammatory cytokines and microbial stimuli, and it functions primarily through CXCR1 and CXCR2 receptors to promote neutrophil recruitment, activation, and chemotaxis at sites of infection or tissue injury. In healthy ferrets, circulating CXCL6 levels are typically low or near the limit of detection (often <10-20 pg/mL in serum or plasma), but concentrations can increase to tens or hundreds of pg/mL during acute respiratory viral infections, bacterial co-infections, airway inflammation, or experimental immune stimulation, depending on disease severity and timing of sampling. In translational and infectious disease research, particularly in ferret respiratory virus models such as influenza and coronaviruses, ferret CXCL6 serves as an important biomarker of neutrophil trafficking, innate immune activation, and inflammatory lung responses. The ELISA kit contains a capture antibody, detection antibody, and recombinant ferret CXCL6 protein standard validated for use together in an ELISA format; kit components are not supplied in matched quantities and may be purchased separately. This product is for research use only, not for use in diagnostic or therapeutic procedures, and is manufactured in the USA.
Storage Conditions2°C to 8°C or -20°C
Country Of OriginUSA
Ferret CXCL6 (C-X-C motif chemokine ligand 6), also known as GCP-2 (granulocyte chemotactic protein 2), is a proinflammatory chemokine belonging to the ELR⁺ CXC chemokine family, which includes CXCL1, CXCL2, CXCL3, and CXCL5 that regulate neutrophil recruitment during inflammatory responses. In ferrets (Mustela putorius furo), CXCL6 is produced by macrophages, epithelial cells, endothelial cells, and fibroblasts in response to proinflammatory cytokines such as IL-1β and TNF-α, microbial infection, or tissue injury. CXCL6 primarily signals through the chemokine receptor CXCR2, which is expressed on neutrophils and other innate immune cells, promoting chemotaxis, activation, and migration of neutrophils to sites of infection or inflammation. In ferret biology, CXCL6 is particularly relevant to respiratory immune responses, as ferrets are widely used as animal models for respiratory viral infections, including influenza virus and SARS-CoV-2. In these models, CXCL6 contributes to neutrophil recruitment and inflammatory signaling within lung tissues, helping regulate the innate immune response to respiratory pathogens. Consequently, ferret CXCL6 is studied in infectious disease and respiratory inflammation research, aiding in the understanding of neutrophil-driven immune responses and host–pathogen interactions in respiratory disease models relevant to human and veterinary medicine.

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