Caprine CXCL10 (IP-10) ELISA

Catalog Number:
DIY2203G
Availability:
In stock
Application:
ELISA
100% Homology:
Capra hircus (goat)

Components

UsageQuantityComponentSize
Detection Antibody50 µgKPB2202G-050
Standard5 µgRP1189G-005
Capture Antibody100 µgKP2201G-1001 Pack (<= 10 Plates)
Capture Antibody100 µg X 2KP2201G-1001 Pack (<= 20 Plates)
  • The Caprine CXCL10 (C-X-C motif chemokine ligand 10, also known as IP-10) ELISA is designed for the quantitative measurement of CXCL10 in goat (Capra hircus) cell culture supernatants and can also be used to measure CXCL10 in additional biological sample types reported in the literature, including serum, plasma, milk, bronchoalveolar lavage (BAL) fluid, and tissue lysates, with minor protocol modifications recommended to optimize performance across different matrices. CXCL10 is an interferon-inducible CXC chemokine produced primarily by monocytes, macrophages, endothelial cells, and epithelial cells in response to interferon-γ (IFN-γ) and type I interferons, and it functions through the CXCR3 receptor to recruit activated T lymphocytes and natural killer (NK) cells to sites of inflammation. In healthy goats, circulating CXCL10 levels are typically low or near the limit of detection, but concentrations can increase substantially during viral or bacterial infections, respiratory disease, mastitis, enteric inflammation, parasitic challenge, or experimental immune stimulation, depending on disease severity and timing of sampling. In veterinary and translational research, caprine CXCL10 serves as an important biomarker of Th1-type immune activation, interferon-driven inflammatory responses, host-pathogen interactions, and vaccine-induced cellular immunity in small ruminant disease models. The ELISA kit contains a capture antibody, detection antibody, and recombinant caprine CXCL10 protein standard validated for use together in an ELISA format; kit components are not supplied in matched quantities and may be purchased separately. This product is for research use only, not for use in diagnostic or therapeutic procedures, and is manufactured in the USA.
Storage Conditions2°C to 8°C or -20°C
Country Of OriginUSA
Caprine CXCL10 (C-X-C motif chemokine ligand 10), also known as IP-10 (Interferon gamma–induced protein 10), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL9 (MIG) and CXCL11 (I-TAC) that regulate T-cell recruitment during antiviral and inflammatory immune responses. In goats (Capra hircus), CXCL10 is produced by macrophages, dendritic cells, endothelial cells, epithelial cells, and fibroblasts in response to interferon-γ (IFN-γ), viral infection, or inflammatory cytokines. CXCL10 signals primarily through the chemokine receptor CXCR3, which is expressed on activated T lymphocytes, natural killer (NK) cells, and other immune cells, promoting chemotaxis and activation of these immune cells at sites of infection or inflammation. In caprine health, CXCL10 contributes to immune responses against viral and bacterial pathogens, including infections such as caprine arthritis encephalitis virus (CAEV) and Mycobacterium avium subsp. paratuberculosis, where recruitment of Th1-type T cells is important for controlling intracellular pathogens. Because these infections involve cell-mediated immune responses similar to those seen in related human and livestock diseases, caprine CXCL10 is studied in veterinary immunology and host–pathogen interaction research, helping to clarify mechanisms of interferon-driven immune responses and T-cell trafficking in small ruminants.

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