Bovine IL-6 Polyclonal Antibody

Catalog Number:: KP0652B
Availability:: In stock
Application:: ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
Host:: Rabbit
100% Homology:: Bos indicus (zebu), Bos javanicus (banteng), Bos taurus (cattle)
Label:: Unconjugated

Anti-bovine IL-6 polyclonal antibody (catalog KP0652B) is made in chickens, affinity-purified by bovine IL-6 affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant bovine IL-6 (immunogen) and the calculated molecular weight of bovine IL-6 is ~20.7 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 1-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody KP0652B; standard protein RP0014B-005; detection antibody KPB0653B-050; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows moderate reactivity to caprine and ovine IL-6 and no reactivity to Atlantic salmon, bat, canine, chicken, cynomolgus monkey, dolphin, equine, feline, ferret, guinea pig, human, mouse, rabbit, rat, swine or zebrafish IL-6. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.

Bovine Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine and a central member of the IL-6 cytokine family, which includes IL-6, IL-11, IL-27, IL-31, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and cardiotrophin-like cytokine factor 1 (CLCF1), all of which signal through receptor complexes utilizing the shared gp130 subunit. In cattle (Bos taurus), IL-6 plays a critical role in regulating acute-phase protein synthesis (including haptoglobin and serum amyloid A), B- and T-cell differentiation, hematopoiesis, and systemic inflammatory responses via the IL-6R/gp130-mediated JAK/STAT pathway. It is a key mediator in economically significant diseases such as bovine respiratory disease (BRD), mastitis, metritis, Johne's disease, and other bacterial and viral infections, where elevated IL-6 levels correlate with fever, tissue inflammation, and disease severity. As a biomarker, bovine IL-6 is widely used to assess immune activation, monitor herd health, and evaluate vaccine efficacy or therapeutic interventions. In animal model and translational research, characterization of bovine IL-6 and related family members supports studies of host-pathogen interactions, inflammatory regulation in large-animal systems, comparative immunology, and the development of immunomodulatory strategies to improve livestock health, productivity, and welfare.

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