Equine IFN alpha 1 Polyclonal Antibody

Anti-equine IFN-α1 polyclonal antibody (catalog KP0887E) is made in rabbits, affinity-purified (equine IFN-α1 affinity chromatography) and supplied in PBS containing 0.09% NaN₃. The antibody was raised against recombinant equine IFN-α1 (immunogen); the calculated molecular weight of equine IFN-α1 is ~18.5 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 0.5-5 µg/mL (optimize per assay). Typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody KP0887E-100; standard protein RP0142E-005; detection antibody KPB0888E-050; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows weak reactivity to recombinant bovine and canine IFN-α, moderate reactivity to recombinant swine IFN-α1, and no reactivity to recombinant chicken IFN-α. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should optimize and validate conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.

Equine Interferon-Alpha (IFN-α) is a type I interferon produced primarily by plasmacytoid dendritic cells and virus-infected epithelial and immune cells in horses (Equus caballus), where it plays a central role in early innate antiviral defense. Upon binding to the type I interferon receptor complex (IFNAR1/IFNAR2), equine IFN-α activates JAK/STAT signaling pathways, leading to induction of interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote activation of natural killer (NK) cells and adaptive immune responses. IFN-α is rapidly upregulated in response to important equine viral pathogens such as equine influenza virus, equine herpesvirus-1 and -4 (EHV-1/EHV-4), West Nile virus, and equine arteritis virus, where it contributes to viral control but may also influence inflammatory pathology if dysregulated. Modulation of type I interferon responses can affect disease severity, viral shedding, and vaccine responsiveness. In veterinary and translational research, characterization of equine IFN-α supports studies of respiratory viral pathogenesis, neuroinvasive viral disease (e.g., EHV-1 and West Nile virus), antiviral therapeutic development, and comparative type I interferon biology in large-animal systems relevant to both equine health and broader immunological research.

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