Swine CCL2 (MCP-1) Polyclonal Antibody

Catalog Number:: PB0088S
Availability:: In stock
Application:: ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
Host:: Rabbit
100% Homology:: Sus scrofa (pig)
Label:: Unconjugated

Product Description

Anti-swine CCL2 (MCP-1) polyclonal antibody (catalog PB0088S) is made in rabbits, affinity-purified by swine CCL2 affinity chromatography and supplied in 1X Tris buffer containing 0.09% NaN₃; it was raised against recombinant swine CCL2 (immunogen) and the calculated molecular weight of swine CCL2 is ~8.9 kDa. Store at 2-8°C (stable up to twelve months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 0.5-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0088S; standard protein RP0017S-005; detection antibody PBB0089S; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows strong reactivity to bovine, canine and equine CCL2; weak reactivity to guinea pig and mouse CCL2; and no reactivity to rabbit CCL2. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.

Properties

Storage Conditions	2°C to 8°C
Purification	Antigen-affinity
Country Of Origin	USA

Background Info

Swine CCL2 (C-C motif chemokine ligand 2, also known as monocyte chemoattractant protein-1, MCP-1) is a pro-inflammatory chemokine that plays an important role in recruitment and activation of monocytes, macrophages, and memory T cells during immune responses in pigs (Sus scrofa domesticus). CCL2 signals primarily through the CCR2 receptor, activating intracellular pathways that promote leukocyte migration, cytokine production, and coordination of innate and adaptive immune responses at sites of infection, inflammation, or tissue injury. In swine health, CCL2 is particularly relevant in respiratory and systemic infectious diseases, including porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza, and bacterial pneumonia, where monocyte and macrophage recruitment to the lungs influences both antiviral defense and inflammatory lung pathology. CCL2 is also involved in inflammatory responses in tissues such as the gut and joints, contributing to immune cell infiltration and tissue remodeling. While CCL2-mediated immune cell trafficking supports pathogen clearance and tissue repair, excessive or prolonged expression can contribute to chronic inflammation and tissue damage. Because pigs are widely used as large-animal models for human infectious, inflammatory, and cardiovascular diseases, characterization of swine CCL2 supports research into chemokine-mediated immune regulation, host-pathogen interactions, and development of anti-inflammatory or immunomodulatory therapies relevant to both veterinary and human medicine.

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