Swine IFN beta Polyclonal Antibody

Catalog Number:: PB0124S
Availability:: In stock
Application:: ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
Host:: Rabbit
100% Homology:: Phacochoerus africanus (Common warthog), Potamochoerus larvatus (bushpig), Potamochoerus porcus (red river hog), Sus scrofa (pig)
Label:: Unconjugated

Product Description

Anti-swine IFN-β polyclonal antibody (catalog PB0124S) is made in rabbits, affinity-purified by swine IFN-β affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant swine IFN-β (immunogen) and the calculated molecular weight of swine IFN-β is ~19.5 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 0.5-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0124S; standard protein RP0011S-005; detection antibody KPB1064S; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows weak reactivity to bovine, canine, equine and mouse IFN-β. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.

Properties

Storage Conditions	2°C to 8°C
Purification	Antigen-affinity
Country Of Origin	USA

Background Info

Swine Interferon-Beta (IFN-β) is a type I interferon produced primarily by virus-infected epithelial cells, fibroblasts, macrophages, and dendritic cells in pigs (Sus scrofa domesticus), where it serves as a key early mediator of innate antiviral immunity. Following recognition of viral nucleic acids by pattern recognition receptors such as RIG-I, MDA5, and the cGAS-STING pathway, swine IFN-β is rapidly induced and signals through the type I interferon receptor complex (IFNAR1/IFNAR2), activating JAK/STAT signaling and driving expression of interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote activation of natural killer (NK) cells and adaptive immune responses. IFN-β plays a critical role in host defense against economically significant viral pathogens including porcine reproductive and respiratory syndrome virus (PRRSV), African swine fever virus (ASFV), swine influenza virus, classical swine fever virus (CSFV), and porcine epidemic diarrhea virus (PEDV). Many of these viruses possess mechanisms to suppress or delay IFN-β production, contributing to immune evasion, viral persistence, and disease severity. The timing and magnitude of IFN-β responses influence viral control, inflammation, and vaccine efficacy. In veterinary and translational research, characterization of swine IFN-β supports studies of antiviral immunity, immune evasion strategies, vaccine development, and comparative type I interferon biology in a large-animal model with physiological similarities to humans, enhancing relevance to both animal health and human infectious disease research.

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