Slide 1 of 1 
Bovine IL-1 alpha Polyclonal Antibody
- Catalog Number:
- PB0331B
- Availability:
- In stock
- Application:
- ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
- Host:
- Rabbit
- 100% Homology:
- Bison bison bison (American buffalo), Bos indicus (zebu), Bos javanicus (banteng), Bos taurus (cattle)
- Label:
- Unconjugated
- Anti-bovine IL-1α polyclonal antibody (catalog PB0331B) is made in rabbits, affinity-purified by bovine IL-1α affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant bovine IL-1α (immunogen) and the calculated molecular weight of bovine IL-1α is ~17.1 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 1-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0331B; standard protein RP0097B-005; detection antibody PBB0332B; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows moderate reactivity to equine IL-1α, weak reactivity to canine and feline IL-1α, and no reactivity to dolphin or rabbit IL-1α. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.
| Storage Conditions | 2°C to 8°C |
| Purification | Antigen-affinity |
| Country Of Origin | USA |
- Bovine Interleukin-1 Alpha (IL-1α) is a pro-inflammatory cytokine belonging to the IL-1 family, which includes IL-1α, IL-1β, and the IL-1 receptor antagonist (IL-1Ra), and plays a central role in initiating and amplifying innate immune responses in cattle (Bos taurus). IL-1α is constitutively expressed in epithelial and barrier tissues and can function as an intracellular alarmin that is released upon cell injury or necrosis, triggering rapid inflammatory signaling. It binds to the IL-1 receptor type 1 (IL-1R1), activating NF-κB and MAPK pathways that promote leukocyte recruitment, endothelial activation, fever, and production of additional pro-inflammatory mediators. In bovine health, IL-1α is particularly relevant in inflammatory conditions such as mastitis, bovine respiratory disease (BRD), metritis, enteric infections, and lameness-associated tissue injury, where it contributes to early neutrophil infiltration and local inflammatory amplification. While essential for pathogen defense and tissue repair, excessive IL-1α signaling can contribute to tissue damage and reduced productivity. As an early mediator of sterile and infectious inflammation, bovine IL-1α serves as a biomarker of tissue injury and innate immune activation. In veterinary and translational research, characterization of bovine IL-1α supports studies of inflammasome-independent inflammatory pathways, host-pathogen interactions, and development of targeted anti-inflammatory or immunomodulatory strategies to improve cattle health, welfare, and disease resilience.
Please choose a size to see price
