Equine IL-8 Polyclonal Antibody

Catalog Number:: PB0433E
Availability:: In stock
Application:: ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
Host:: Goat
100% Homology:: Equus caballus (horse), Equus przewalskii (Przewalski's horse)
Label:: Unconjugated

Anti-equine IL-8 polyclonal antibody (catalog PB0433E) is made in goats, affinity-purified by equine IL-8 affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant equine IL-8 (immunogen) and the calculated molecular weight of equine IL-8 is ~8.9 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 0.5-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0433E; standard protein RP0312E-005; detection antibody PBB0434E; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows no reactivity to bovine, canine, dolphin, guinea pig or human IL-8 and weak reactivity to feline IL-8. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.

Equine Interleukin-8 (IL-8), also known as CXCL8, is a potent pro-inflammatory chemokine that plays a central role in innate immune responses in horses (Equus caballus) by mediating neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Produced by macrophages, respiratory and intestinal epithelial cells, endothelial cells, and synoviocytes in response to microbial stimuli and inflammatory cytokines, equine IL-8 signals primarily through CXCR1 and CXCR2 receptors to promote rapid neutrophil recruitment and amplification of acute inflammation. Elevated IL-8 expression is associated with common equine conditions such as recurrent airway obstruction (equine asthma), bacterial pneumonia, strangles (Streptococcus equi infection), colitis, endotoxemia, osteoarthritis, and laminitis, where excessive neutrophilic inflammation can contribute to tissue damage and disease severity. As a biomarker, IL-8 is valuable for assessing inflammatory status, monitoring respiratory and joint disease progression, and evaluating treatment responses. In research settings, horses serve as important large-animal models for human asthma, osteoarthritis, sepsis, and exercise-induced inflammation, and characterization of equine IL-8 enhances understanding of neutrophil-driven pathology, mucosal immune responses, and chemokine-targeted therapeutic strategies relevant to both veterinary and human medicine.

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