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Guinea Pig TNF alpha Polyclonal Antibody
- Catalog Number:
- PB0446GP
- Availability:
- In stock
- Application:
- ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
- Host:
- Goat
- 100% Homology:
- Cavia porcellus (domestic guinea pig)
- Label:
- Unconjugated
- Anti-guinea pig TNF-α polyclonal antibody (catalog PB0446GP) is made in goats, affinity-purified (guinea pig TNF-α affinity chromatography) and supplied in PBS containing 0.09% NaN₃. The antibody was raised against recombinant guinea pig TNF-α (immunogen). The calculated molecular weight of guinea pig TNF-α is ~17.0 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 1-5 µg/mL (optimize per assay). Typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0446GP-100; standard protein RP0345GP-005; detection antibody PBB0452GP-050; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows no reactivity to bovine, canine, dolphin, equine, feline, human or swine TNF-α, and weak reactivity to mouse, ovine and rabbit TNF-α. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should optimize and validate conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.
| Storage Conditions | 2°C to 8°C |
| Purification | Antigen-affinity |
| Country Of Origin | USA |
- Guinea Pig Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in guinea pigs (Cavia porcellus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, proteins that regulate immune signaling, apoptosis, and inflammatory pathways. In guinea pigs, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In guinea pigs, TNF-α is particularly important in immune responses to intracellular bacterial infections such as Mycobacterium tuberculosis, for which guinea pigs serve as a well-established animal model of human tuberculosis due to their similar disease pathology and granuloma formation. TNF-α contributes to macrophage activation, granuloma maintenance, and containment of mycobacterial infection, although excessive TNF signaling can also contribute to tissue inflammation and pathology. Because guinea pigs are widely used in infectious disease, immunology, and tuberculosis research, characterization of guinea pig TNF-α supports studies of cytokine regulation, host-pathogen interactions, and inflammatory responses relevant to both veterinary and human health.
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