Slide 1 of 1 
Equine IL-1 alpha Polyclonal Antibody
- Catalog Number:
- PB0471E
- Availability:
- In stock
- Application:
- ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
- Host:
- Goat
- 100% Homology:
- Equus caballus (horse)
- Label:
- Unconjugated
- Anti-equine IL-1α polyclonal antibody (catalog PB0471E) is made in goats, affinity-purified by equine IL-1α affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant equine IL-1α (immunogen) and the calculated molecular weight of equine IL-1α is ~18.1 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 1-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0471E; standard protein RP0358E-005; detection antibody PBB0480E; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows moderate reactivity to bovine IL-1α, strong reactivity to canine and feline IL-1α, and no reactivity to dolphin, mouse or rabbit IL-1α. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.
| Storage Conditions | 2°C to 8°C |
| Purification | Antigen-affinity |
| Country Of Origin | USA |
- Equine Interleukin-1 Alpha (IL-1α) is a pro-inflammatory cytokine belonging to the IL-1 family-which includes IL-1α, IL-1β, and the IL-1 receptor antagonist (IL-1Ra)-and plays a pivotal role in initiating innate immune responses in horses (Equus caballus). IL-1α is constitutively expressed in epithelial and barrier tissues and acts as an intracellular alarmin that is rapidly released upon cell injury, necrosis, or mechanical stress, triggering early inflammatory signaling. Upon binding to the IL-1 receptor type 1 (IL-1R1), IL-1α activates NF-κB and MAPK pathways that promote leukocyte recruitment, endothelial activation, fever, and amplification of pro-inflammatory cytokine cascades. In equine health, IL-1α is particularly important in musculoskeletal inflammation such as osteoarthritis and synovitis, where it contributes to cartilage matrix degradation and synovial inflammation, as well as in laminitis, colitis, endotoxemia, and respiratory inflammatory conditions. While critical for initiating protective immune responses and tissue repair, excessive or sustained IL-1α signaling can exacerbate tissue damage and systemic inflammatory responses. As an early mediator of sterile and infectious inflammation, equine IL-1α serves as a biomarker of tissue injury and innate immune activation. In veterinary and translational research, characterization of equine IL-1α supports studies of joint disease pathogenesis, systemic inflammatory response syndrome (SIRS), cytokine regulation, and development of targeted anti-inflammatory and regenerative therapies relevant to both equine and human inflammatory diseases.
Please choose a size to see price
