Rabbit CCL2 (MCP-1) Polyclonal Antibody

Catalog Number:
PB0911U
Availability:
In stock
Application:
ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
Host:
Goat
100% Homology:
Oryctolagus cuniculus (rabbit)
Label:
Unconjugated
  • Anti-rabbit CCL2 (MCP-1) polyclonal antibody (catalog PB0911U) is made in goats, affinity-purified by rabbit CCL2 affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant rabbit CCL2 (immunogen) and the calculated molecular weight of rabbit CCL2 is ~11.4 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 0.5-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0911U; standard protein RP0431U-005; detection antibody PBB0912U; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows weak reactivity to bovine, canine, equine, mouse and swine CCL2, and no reactivity to guinea pig CCL2. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.
Storage Conditions2°C to 8°C
PurificationAntigen-affinity
Country Of OriginUSA
Rabbit CCL2 (C-C motif chemokine ligand 2, also known as monocyte chemoattractant protein-1, MCP-1) is a pro-inflammatory chemokine that regulates recruitment and activation of monocytes, macrophages, and memory T cells during immune responses in rabbits (Oryctolagus cuniculus). CCL2 signals primarily through the CCR2 receptor, activating intracellular pathways that promote leukocyte migration, cytokine production, and coordination of innate and adaptive immune responses at sites of infection, tissue injury, or inflammation. In rabbit health and research, CCL2 is particularly important in inflammatory and cardiovascular disease models, including atherosclerosis, where macrophage recruitment to vascular lesions contributes to plaque formation and progression. CCL2 is also involved in infectious disease and pulmonary inflammation models, influencing immune cell infiltration and tissue remodeling during bacterial or viral infections. While CCL2-mediated monocyte recruitment supports host defense and tissue repair, excessive or prolonged expression can promote chronic inflammation and tissue damage. Because rabbits are widely used as translational models for cardiovascular disease, inflammatory disorders, and respiratory pathology, characterization of rabbit CCL2 supports studies of chemokine-mediated immune cell trafficking, host–pathogen interactions, and inflammatory mechanisms relevant to human disease.

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