Rabbit CXCL10 (IP-10) Polyclonal Antibody

Catalog Number:
PB0914U
Availability:
In stock
Application:
ELISA, ELISpot, Flow Cytometry, Neutralization, Western Blot
Host:
Goat
100% Homology:
Oryctolagus cuniculus (rabbit)
Label:
Unconjugated
  • Anti-rabbit CXCL10 (IP-10) polyclonal antibody (catalog PB0914U) is made in goats, affinity-purified by rabbit CXCL10 affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant rabbit CXCL10 (immunogen) and the calculated molecular weight of rabbit CXCL10 is ~8.6 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). Recommended working concentrations are Western blot 0.1-2 µg/mL and ELISA 0.5-5 µg/mL (optimize per assay); typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0914U; standard protein RP0455U-005; detection antibody PBB0915U; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows weak reactivity to canine, dolphin, equine, feline, human, mouse and swine CXCL10, and no reactivity to bovine, caprine, cynomolgus monkey, guinea pig or ovine CXCL10. It is commonly used for ELISA, ELISpot, flow cytometry, neutralization, and Western blot applications (users should validate and optimize conditions for each application). The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.
Storage Conditions2°C to 8°C
PurificationAntigen-affinity
Country Of OriginUSA
Rabbit CXCL10 (C-X-C motif chemokine ligand 10), also known as IP-10 (Interferon gamma–induced protein 10), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL9 (MIG) and CXCL11 (I-TAC) that regulate T-cell recruitment during antiviral and inflammatory immune responses. In rabbits (Oryctolagus cuniculus), CXCL10 is produced by macrophages, dendritic cells, epithelial cells, endothelial cells, and fibroblasts in response to interferon-γ (IFN-γ), viral infection, or inflammatory cytokines. CXCL10 signals primarily through the chemokine receptor CXCR3, expressed on activated T lymphocytes, natural killer (NK) cells, and other immune cells, promoting chemotaxis and activation of these cells at sites of infection or inflammation. In rabbits, CXCL10 plays an important role in Th1-type immune responses, particularly during viral infections and inflammatory diseases. Rabbits are widely used as animal models for atherosclerosis and vascular inflammation, where CXCL10 contributes to recruitment of CXCR3⁺ T cells and other leukocytes to inflamed vascular tissue, influencing immune-mediated vascular pathology and plaque development. Consequently, rabbit CXCL10 is studied in immunology, cardiovascular disease research, and host–pathogen interaction studies to better understand chemokine-driven immune cell trafficking and inflammatory disease mechanisms.

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