Bovine CCL4 (MIP-1 beta) Polyclonal Antibody - Biotinylated

Catalog Number:: PBB0479B
Availability:: In stock
Application:: ELISA, ELISpot
Host:: Goat
100% Homology:: Bison bison bison (American buffalo), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle)
Label:: Biotin

Biotinylated Anti-bovine CCL4 (alias MIP-1β) polyclonal antibody (catalog PBB0479B) is a biotinylated, made-in-goats reagent, affinity-purified by bovine CCL4 affinity chromatography and supplied in PBS containing 0.09% NaN₃; it was raised against recombinant bovine CCL4 (immunogen) and the calculated molecular weight of bovine CCL4 is ~7.8 kDa. Store at 2-8°C (stable up to 12 months from date of receipt). The antibody is supplied for ELISA (recommended 0.01-0.5 µg/mL; optimize per assay) and typical sandwich ELISA reagent pairings and suggested conditions are provided on the datasheet (capture antibody PB0470B-100; standard protein RP0361B-005; detection antibody PBB0479B; streptavidin-HRP AR0068-001; TMB substrate AR0133-002). In cross-reactivity testing (ELISA) this antibody shows strong reactivity to canine, rabbit, rat and swine CCL4; moderate reactivity to equine and human CCL4; weak reactivity to dolphin CCL4; and no reactivity to chicken or mouse CCL4. It is commonly used for ELISA and ELISpot. The product is made in the USA, supplied for research applications only, and is not intended for medicinal, diagnostic, or therapeutic use.

Bovine CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in cattle (Bos taurus). CCL4 primarily signals through the CCR5 receptor, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In cattle, CCL4 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL4-mediated immune cell recruitment plays an important role in host defense against pathogens involved in diseases such as bovine respiratory disease complex (BRDC), mastitis, and mycobacterial infections including Mycobacterium bovis. In these conditions, infiltration of monocytes and lymphocytes contributes to pathogen clearance and activation of adaptive immune responses. However, excessive or prolonged expression of CCL4 may contribute to inflammatory tissue damage and chronic inflammation. As a regulator of leukocyte trafficking and inflammatory signaling, bovine CCL4 is important for studies of host-pathogen interactions, inflammatory disease mechanisms, and vaccine responses aimed at improving immune protection and disease resistance in cattle.

Choose a size

Price: $400.00

RELATED MEDIA