Bovine IL-4 Recombinant Protein

Catalog Number:: RP0001B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bison bonasus (European bison), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle), Bubalus bubalis (water buffalo), Bubalus kerabau (carabao), Syncerus caffer (African buffalo)

Product Description

Bovine IL-4 (catalog RP0001B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.6 kDa, 112 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species: cattle, American buffalo, European bison, water buffalo, carabao, wild yak, African buffalo, and zebu. Store at -20°C (stable up to 12 months from receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study IL-4 signaling and Th2 immune responses, to promote B-cell proliferation and antibody class switching (including IgE), to drive differentiation of naive CD4⁺ T cells toward a Th2 phenotype, to induce alternative (M2) macrophage activation, and to support mast cell and eosinophil function; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	GHKCDIT LAEIIKTLNI LTTRKNSCME LPVADVFAAP KNTTEKETFC RVGIELRRIY RSHTCLNKFL GGLDRNLNSL ASKTCSVNEA KTSTSTLKDL LERLKTIMKE KYSKC (115)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	12.6kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine Interleukin-4 (IL-4) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and basophils in cattle (Bos taurus), where it plays a central role in regulating humoral immunity, alternative macrophage activation, and modulation of inflammatory responses. IL-4 signals through receptor complexes containing IL-4Rα paired with the common gamma chain (γc) or IL-13Rα1, activating JAK/STAT6 pathways that promote B-cell proliferation, antibody class switching, and suppression of Th1-associated cytokines such as IFN-γ. In bovine health, IL-4 is particularly important in responses to parasitic infections-including gastrointestinal nematodes and lungworms-where Th2 polarization supports parasite clearance and mucosal repair. IL-4 also influences immune balance during infections such as bovine viral diarrhea virus (BVDV), mastitis, and respiratory disease by shaping Th1/Th2 dynamics, which can affect pathogen control and disease outcome. While IL-4-mediated responses are protective in parasite defense and tissue repair, excessive Th2 skewing may impair effective cell-mediated immunity against intracellular pathogens. As a biomarker of type 2 immune activation, bovine IL-4 is relevant in vaccine evaluation, host-parasite interaction studies, and immune resilience research. Characterization of bovine IL-4 supports veterinary investigations into cytokine network regulation, disease susceptibility, and strategies to enhance cattle health and productivity through balanced immune modulation.

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