Equine IL-4 Recombinant Protein

Catalog Number:: RP0003E
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Equus asinus (ass), Equus caballus (horse), Equus przewalskii (Przewalski's horse), Equus zebra hartmannae (Hartmann's mountain zebra)

Product Description

Equine IL-4 (catalog RP0003E) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.8 kDa, 114 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several equid species: horse, Przewalski's horse, donkey, and Hartmann's mountain zebra. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study IL-4 signaling and Th2 immune responses, to promote B-cell proliferation and antibody class switching (including IgE), to drive differentiation of naive CD4⁺ T cells toward a Th2 phenotype, to induce alternative (M2) macrophage activation, and to support mast cell and eosinophil function; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	GCKYDITLQE IIKTLNNLTD GKGKNSCMEL TVADAFAGPK NTDGKEICRA AKVLQQLYKR HDRSLIKECL SGLDRNLKGM ANGTCCTVNE AKKSTLKDFL ERLKTIMKEK YSKC (114)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	12.8kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Equine Interleukin-4 (IL-4) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and basophils in horses (Equus caballus), where it plays a central role in regulating humoral immunity, allergic inflammation, and alternative macrophage activation. IL-4 signals through receptor complexes containing IL-4Rα paired with the common gamma chain (γc) or IL-13Rα1, activating JAK/STAT6 pathways that promote B-cell proliferation, antibody production (including IgE-associated responses), and suppression of Th1-associated cytokines such as IFN-γ. In equine health, IL-4 is particularly important in allergic and hypersensitivity conditions such as equine asthma (recurrent airway obstruction), insect bite hypersensitivity ("sweet itch"), and certain forms of inflammatory bowel disease, where Th2 polarization contributes to eosinophilic inflammation, airway hyperreactivity, and pruritic skin lesions. IL-4 also influences immune responses to parasitic infections by supporting humoral immunity and mucosal repair. While protective in promoting antibody-mediated defense and limiting excessive inflammation, dysregulated or persistent IL-4 signaling may contribute to chronic allergic disease and tissue remodeling. As a biomarker of type 2 immune activation, equine IL-4 is relevant in vaccine development, allergy research, and immune modulation strategies. In translational research, horses serve as valuable large-animal models for human asthma and allergic airway disease, and characterization of equine IL-4 supports comparative investigations into Th2-mediated inflammation and development of targeted cytokine therapies relevant to both veterinary and human medicine.

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