Swine IFN beta Recombinant Protein

Catalog Number:: RP0011S
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Phacochoerus africanus (Common warthog), Potamochoerus larvatus (bushpig), Potamochoerus porcus (red river hog), Sus scrofa (pig)

Product Description

Swine IFN-β (catalog RP0011S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.5 kDa, 164 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across pig, common warthog, bushpig, and red river hog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study interferon signaling and antiviral responses, induction of interferon-stimulated genes and antiviral states, and for antiviral/cytopathic-effect assays; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	SYDVLRYQQR SSNLACQKLL GQLPGTPQYC LEDRMNFEVP EEIMQPPQFQ KEDAVLIIHE MLQQIFGILR RNFSSTGWNE TVIKTILVEL DGQMDDLETI LEEIMEEENF PRGDMTILHL KKYYLSILQY LKSKEYRSCA WTVVQVEILR NFSFLNRLTD YLRN (164)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	19.5 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Swine Interferon-Beta (IFN-β) is a type I interferon produced primarily by virus-infected epithelial cells, fibroblasts, macrophages, and dendritic cells in pigs (Sus scrofa domesticus), where it serves as a key early mediator of innate antiviral immunity. Following recognition of viral nucleic acids by pattern recognition receptors such as RIG-I, MDA5, and the cGAS-STING pathway, swine IFN-β is rapidly induced and signals through the type I interferon receptor complex (IFNAR1/IFNAR2), activating JAK/STAT signaling and driving expression of interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote activation of natural killer (NK) cells and adaptive immune responses. IFN-β plays a critical role in host defense against economically significant viral pathogens including porcine reproductive and respiratory syndrome virus (PRRSV), African swine fever virus (ASFV), swine influenza virus, classical swine fever virus (CSFV), and porcine epidemic diarrhea virus (PEDV). Many of these viruses possess mechanisms to suppress or delay IFN-β production, contributing to immune evasion, viral persistence, and disease severity. The timing and magnitude of IFN-β responses influence viral control, inflammation, and vaccine efficacy. In veterinary and translational research, characterization of swine IFN-β supports studies of antiviral immunity, immune evasion strategies, vaccine development, and comparative type I interferon biology in a large-animal model with physiological similarities to humans, enhancing relevance to both animal health and human infectious disease research.

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