Bovine IFN gamma Recombinant Protein

Catalog Number:: RP0013B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos frontalis (gayal), Bos taurus (cattle)

Product Description

Bovine IFN-γ (catalog RP0013B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.9 kDa, 143 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across cattle and gayal. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study IFN-γ signaling, macrophage activation, Th1 immune responses, antigen presentation and immunoregulatory functions; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	QGQFFREIEN LKEYFNASSP DVAKGGPLFS EILKNWKDES DKKIIQSQIV SFYFKLFENL KDNQVIQRSM DIIKQDMFQK FLNGSSEKLE DFKKLIQIPV DDLQIQRKAI NELIKVMNDL SPKSNLRKRK RSQNLFRGRR AST (143)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	16.9 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine Interferon-Gamma (IFN-γ) is a type II interferon produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes and natural killer (NK) cells in cattle (Bos taurus), where it plays a central role in cell-mediated (Th1-type) immunity against intracellular pathogens. IFN-γ signals through the IFN-γ receptor complex (IFNGR1/IFNGR2), activating JAK/STAT1 pathways and inducing genes involved in macrophage activation, enhanced antigen presentation via upregulation of MHC class I and II molecules, nitric oxide production, and promotion of cytotoxic T-cell responses. In bovine health, IFN-γ is critically important for defense against economically significant intracellular infections such as Mycobacterium bovis (bovine tuberculosis), Mycobacterium avium subsp. paratuberculosis (Johne's disease), Brucella abortus, and certain viral pathogens including bovine viral diarrhea virus (BVDV). Measurement of antigen-specific IFN-γ release forms the basis of widely used diagnostic assays for bovine tuberculosis and serves as an indicator of cellular immune activation and vaccine responsiveness. Dysregulated or insufficient IFN-γ responses can contribute to chronic infection, pathogen persistence, and altered disease progression. In veterinary and translational research, characterization of bovine IFN-γ supports studies of host-pathogen interactions, Th1/Th2 immune balance, vaccine efficacy, and immune correlates of protection, while also providing a valuable large-animal model for understanding cell-mediated immunity relevant to human tuberculosis and other intracellular infectious diseases.

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