Chicken IL-10 Recombinant Protein

Catalog Number:: RP0018C
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Gallus gallus (chicken)

Product Description

Chicken IL-10 (catalog RP0018C) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.3 kDa, 154 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to chicken. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study IL-10 signaling and anti-inflammatory/regulatory functions (macrophage/monocyte regulation, Treg biology, suppression of pro-inflammatory cytokines); typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry controls, signaling assays, and antibody generation/validation.

Specifications

Amino Acid Sequence	LEPTCLHFSE LLPARLRELR VKFEEIKDYF QSRDDELNIQ LLSSELLDKF KGTFGCQSVS EMLRFYTDEV LPRAMQTSTS HQQSMGDLGN MLLGLKATMR RCHRFFTCEK RSKAIKQIKE TFEKMDENGI YKAMGEFDIF INYIEEYLLM RRRK (154)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	18.3 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Chicken Interleukin-10 (IL-10) is a key anti-inflammatory cytokine produced primarily by regulatory T cells, Th2-like CD4⁺ T cells, macrophages, dendritic cells, and certain B-cell populations in chickens (Gallus gallus), where it plays a central role in limiting excessive immune activation and maintaining immune homeostasis. IL-10 signals through the IL-10 receptor complex (IL-10R1/IL-10R2), activating JAK/STAT3 pathways that suppress pro-inflammatory cytokine production (including IL-1β, IL-6, and IFN-γ), reduce antigen presentation, and downregulate Th1-type cell-mediated responses. In poultry health, IL-10 is critically involved in modulating immune responses during infections such as avian influenza virus, Newcastle disease virus, infectious bursal disease virus, Marek's disease virus, Salmonella spp., and Eimeria (coccidiosis), where elevated IL-10 can help limit tissue damage but may also contribute to immune suppression and pathogen persistence. The balance between IL-10 and pro-inflammatory cytokines influences vaccine efficacy, disease resistance, and growth performance in commercial flocks. As a biomarker of immune regulation, chicken IL-10 is widely studied in vaccine development, genetic selection for disease resilience, and investigations of mucosal immunity. Characterization of chicken IL-10 supports research into cytokine network regulation, host-pathogen interactions, and comparative immune tolerance mechanisms relevant to both poultry production and broader vertebrate immunology.

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