Bovine IL-8 Recombinant Protein

Catalog Number:: RP0023B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos indicus (zebu), Bos taurus (cattle)

Bovine IL-8 (catalog RP0023B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.1 kDa, 79 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across cattle and zebu. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study chemokine signaling, neutrophil and leukocyte chemoattraction/activation, and inflammation; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry controls, and antibody generation/validation.

Amino Acid Sequence	AVLSRMSTEL RCQCIKTHST PFHPKFIKEL RVIESGPHCE NSEIIVKLTN GNEVCLNPKE KWVQKVVQVF VKRAEKQDP (79)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	9.1kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine Interleukin-8 (IL-8), also known as CXCL8, is a potent pro-inflammatory chemokine that plays a central role in innate immune responses in cattle (Bos taurus) by mediating neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Produced by macrophages, epithelial cells, endothelial cells, and mammary gland cells in response to bacterial components, viral infection, and inflammatory cytokines, bovine IL-8 signals primarily through CXCR1 and CXCR2 receptors to activate downstream pathways that promote leukocyte recruitment and amplification of acute inflammation. IL-8 is especially important in economically significant conditions such as mastitis, bovine respiratory disease (BRD), endometritis, and enteric infections, where elevated IL-8 levels drive neutrophil infiltration into affected tissues, contributing to both pathogen clearance and inflammatory tissue damage. In the mammary gland, IL-8 is a key mediator of somatic cell recruitment during intramammary infection and serves as an early biomarker of mastitis severity and treatment response. In animal health and translational research, characterization of bovine IL-8 supports studies of mucosal immunity, host-pathogen interactions, inflammatory regulation, and genetic factors influencing disease resistance, while also providing comparative insights into neutrophil-driven inflammatory processes relevant to human respiratory and inflammatory diseases.

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